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European Journal of Plant Pathology

, Volume 108, Issue 6, pp 497–506 | Cite as

Identification of the Beet Cyst Nematode Heterodera schachtii by PCR

  • Saïd Amiri
  • Sergei A. Subbotin
  • Maurice MoensEmail author
Article

Abstract

PCR-RFLPs of ITS-rDNA and PCR with species-specific primers were developed for identification of cysts and juveniles of the beet cyst nematode Heterodera schachtii. Restrictions of PCR product by MvaI or ScrFI distinguish H. schachtii, H. betae, H. trifolii and H. medicaginis. RFLP profiles with eight restriction enzymes for these four nematode species are presented. Based on Internal Transcribed Spacer sequences of populations from several Schachtii group species, a specific primer for H. schachtii was designed, permitting amplification of the target sequence from juveniles and cysts of the beet cyst nematode. A duplex PCR protocol tested with a wide range of nematode samples is described.

diagnostics Heterodera betae H. medicaginis H. trifolii RFLP species-specific primer 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Saïd Amiri
    • 1
  • Sergei A. Subbotin
    • 1
  • Maurice Moens
    • 2
    • 3
    • 3
    Email author
  1. 1.Département de PhytopathologieEcole Nationale d’AgricultureMeknèsMorocco
  2. 2.Laboratory for AgrozoologyUniversity of GentGentBelgium
  3. 3.Crop Protection DepartmentAgricultural Research CentreMerelbekeBelgium

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