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Plant Cell, Tissue and Organ Culture

, Volume 71, Issue 2, pp 117–123 | Cite as

The effect of genotype and explant age on somatic embryogenesis of coffee

  • Diana M. Molina
  • María E. Aponte
  • Hernando Cortina
  • Germán Moreno
Article

Abstract

We demonstrate that embryogenic capacity of coffee (Coffea arabica) depends on the genotype, and that it is a character fixed at the early generations F3 or F4. Therefore, the embryogenic capacity F5 lines can be predicted in a breeding program if it is possible to evaluate the F3 of F4 ancestors, or lines with the same parents on those generations.

Regeneration via somatic embryogenesis of genotypes from a C. arabica cv. Caturra by Timor Hybrid cross were evaluated, to determine the effect of the pedigree, month of leaf collection, as well as its interaction with the genotype. Large variations in embryogenic capacity among genotypes were detected with rates ranging from 4.8 to 72.7. There was association between embryogenic response of the progenies and the progenitor from which they proceeded. Significant differences were also found in embryogenic responses depending on the month of leaf explant collection, as well as a significant interaction of genotype by planting month.

The results are relevant for breeding and tissue culture purposes of coffee because they will allow speed up of the breeding program and save effort by being able to predict embryogenic capacity of a line, based on the response of either its progenitors or the F3 or F4 lines of the same origin. In addition, high embryogenic capacity lines can be considered directly for genetic transformation or for massive multiplication (bioreactors).

coffee embryogenic capacity genealogy progeny 

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References

  1. Aponte ME (1993) Somatic embryogenesis induced by cultured on single media in coffee plants from crosses of Coffea arabica by Timor hybrid. In: Quinzième Colloque Scientifique International sur le Café (pp 82–88). ASICGoogle Scholar
  2. Berthouly M & Michaux-Ferrière NM (1996) High frequency somatic embryogenesis in Coffea canephora: Induction conditions and histological evolution. Plant Cell Tiss. Org. Cult. 44: 169–176Google Scholar
  3. Bieysse D, Gofflot A & Michaux-Ferrière N (1993) Effect of experimental conditions and genotypic variability on somatic embryogenesis in Coffea arabica. Can. J. Bot. 71: 1496–1502Google Scholar
  4. Boxtel JV & Berthouly M (1996) High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell Tiss. Org. Cult. 44: 7–17Google Scholar
  5. Chen THH, Marowitch J & Thompson BG (1987) Genotypic effects on somatic embryogenesis and plant regeneration from callus cultures of alfalfa. Plant Cell Tiss. Org. Cult. 8: 73–81Google Scholar
  6. Close KR & Ludeman LA (1987) The effect of auxin-like plant growth regulators and osmotic regulation on induction of somatic embryogenesis from elite maize inbreds. Plant Sci. 52: 81–89Google Scholar
  7. Duncan DR, Williams ME, Zehr BE & Widholm JM (1985) The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes. Planta 165: 322–332.Google Scholar
  8. Evans DA, Sharp WR & Flick CE (1981) Growth and behavior or cells cultures: embryogenesis and organogenesis. In: Thorpe TA (ed) Plant Tissue Culture. Methods and Applications in Agriculture (pp 45–114). Academic Press, OrlandoGoogle Scholar
  9. Fahey JW, Reed JN, Readdy TL & Pace GM (1986). Somatic embryogenesis from three commercially important inbreds of Zea mays. Plant Cell Rep. 5: 35–38Google Scholar
  10. Gmitter FG & Moore GA (1986) Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: Embryo production, germination, and plant survival. Plant Cell Tiss. Org. Cult. 6: 139–147Google Scholar
  11. Gómez L (1977) Influencia de los factores climáticos sobre la periodicidad de crecimiento del cafeto. Cenicafé 28: 3–17Google Scholar
  12. Hanzel JJ, Miller JP, Brinkman MA & Fendos E (1985) Genotype and media effects on callus formation and regeneration in barley. Crop Sci. 25: 27–31Google Scholar
  13. Kahia JW & Owuor JBO (1990) In vitro propagation of the disease resistant Coffea arabica L. cultivar-Riuru 11. Kenya Coffee 55: 901–905Google Scholar
  14. Maës OC, Chibbar RN, Caswell K, Leung N & Kartha KK (1996) Somatic embryogenesis from isolated scutella of wheat: effects of physical, physiological and genetic factors. Plant Sci. 121: 75–84Google Scholar
  15. Michaux-Ferrière N & Dublin P (1987) Embryogenèse somatique chez Coffea arabica. Induction et développement des cellules embryogènes. In: Douzième Colloque Scientifique International sur le Café (pp 418–425). ASICGoogle Scholar
  16. Mroginski LA & Roca WM (1991) Establecimiento de cultivo de tejidos vegetales in vitro. In: Roca WM & Mroginski LA (eds) Cultivo de Tejidos en la Agricultura: Fundamentos y Aplicaciones (pp 19–40). CIAT, CaliGoogle Scholar
  17. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  18. Orozco FJ, Londoño LC, Gonzalez MT, Marulanda ML & Velez BE (1989) Aplicaciones del cultivo de tejidos en el mejoramiento del cafeto. In: Treizième Colloque Scientifique International sur le Café (pp 749–756). ASICGoogle Scholar
  19. Pacheco M & Buitrago HL (1984) Adaptación de plantas de C. arabica cv. Mundo novo obtenidas por embriogénesis somática a cultivo bajo condiciones de campo. Cenicafé 35: 66–76Google Scholar
  20. Purushotham K, Sulladmath UV & Ramaiah PK (1984) Seasonal changes in biochemical constituents and their relation to rooting of coffee (Coffea canephora Pierre) sucker cuttings. J. Coffee Res. 14: 117–130Google Scholar
  21. Rangaswamy NS (1986) Somatic embryogenesis in angiosperm cell tissue and organ cultures. Proc. Indian Acad. Sci. Plant. Sci. 96: 247–271Google Scholar
  22. Rines HW & McCoy TJ (1981) Tissue culture initiation and plant regeneration in hexaploid species of oats. Crop Sci. 21: 837–842Google Scholar
  23. Rodríguez H (1985) Obtención de embriones somáticos in vitro de las variedades comerciales de café: Variedad Caturra y Var-iedad Colombia, BSc Thesis, (pp 1–91). Univ. Javeriana, Bogotá, ColombiaGoogle Scholar
  24. Staritsky G (1970) Embryoid formation in callus tissues of coffee. Acta Bot. Neerl. 19: 509–514Google Scholar
  25. Sondahl MR & Sharp WR (1977) High frequency induction of somatic embryos in cultured leaf explants of Coffea arabica.L.Z. Pflanzenphysiol. 81: 395–408Google Scholar
  26. Yavad RC, Saleh MT & Grumet R (1996) High frequency shoot regeneration from leaf explants of muskmelon. Plant Cell Tiss. Org. Cult. 45: 207–214Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Diana M. Molina
    • 1
  • María E. Aponte
    • 1
  • Hernando Cortina
    • 1
  • Germán Moreno
    • 1
  1. 1.Disciplina de Mejoramiento Genético y BiotecnologíaCentro Nacional de Investigaciones de Café (CENICAFÉ)Plan Alto, Chinchiná, CaldasColombia

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