Plant Molecular Biology

, Volume 50, Issue 4, pp 699–712

Powerful effect of an atypical bifactorial endosperm box from wheat HMWG-Dx5 promoter in maize endosperm

  • Frédéric Norre
  • Corinne Peyrot
  • Christian Garcia
  • Iann Rancé
  • Joel Drevet
  • Manfred Theisen
  • Véronique Gruber
Article

DOI: 10.1023/A:1019953914467

Cite this article as:
Norre, F., Peyrot, C., Garcia, C. et al. Plant Mol Biol (2002) 50: 699. doi:10.1023/A:1019953914467

Abstract

The proximal region of the high-molecular-weight glutenin promoter of the Dx5 gene (PrHMWG-Dx5) carries an atypical bifactorial endosperm box containing two cis-acting elements, namely a G-box like motif followed by a prolamin-box motif (Pb1). Transient expression assays in maize endosperm indicate that a promoter fragment containing at least the G-box like element is necessary and sufficient for maximal expression of the HMWG-Dx5 promoter. In transformed maize, we have shown that a 89 bp sequence bearing the bifactorial endosperm box behaves like a functional cis-acting unit. Its repetition in tandem confers a strong specific additive effect specifically in endosperm tissue. In contrast, the fusion of the activation sequences 1 (as-1) and 2 (as-2) of the cauliflower mosaic virus (CaMV) 35S promoter with HMWG-Dx5 derived promoter sequences deregulates its activity in transformed maize. By gel mobility shift assays we have demonstrated that the G-box like motif may alternatively bind two protein groups which have the same DNA-binding affinities as the transcription factors of either the Opaque2 (O2) family and/or the ASF-1 family.

bifactorial endosperm box HMWG-Dx5 synthetic promoters trans-acting factors transgenic maize 

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Frédéric Norre
    • 1
  • Corinne Peyrot
    • 1
  • Christian Garcia
    • 1
  • Iann Rancé
    • 1
  • Joel Drevet
    • 2
  • Manfred Theisen
    • 1
  • Véronique Gruber
    • 1
  1. 1.MERISTEM TherapeuticsClermont-FerrandFrance
  2. 2.Université Blaise Pascal-Clermont 2, CNRS UMR 6547 GEEMAubière cedexFrance

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