Advertisement

Somatic Cell and Molecular Genetics

, Volume 25, Issue 5–6, pp 301–315 | Cite as

Removal of UV Photoproducts from an Adenovirus-Encoded Reporter Gene Following Infection of Unirradiated and UV-Irradiated Human Fibroblasts

  • Ihor P. Boszko
  • Andrew J. Rainbow
Article

Abstract

Ad5HCMVsp1lacZ is a recombinant nonreplicating adenovirus containing the lacZ gene under the control of the human cytomegalovirus immediate early promoter. Previous reports show that lacZ expression for UV-irradiated Ad5HCMVsp1lacZ is greater in nucleotide excision repair (NER) proficient compared to NER deficient human fibroblasts from patients with xeroderma pigmentosum (XP) and Cockaye's syndrome (CS) and that pre-UV-treatment of normal fibroblasts results in an enhanced expression of the lacZ gene from UV-irradiated Ad5HCMVsp1lacZ. We have used a quantitative PCR technique to examine whether UV photoproducts are actually removed from the lacZ gene following infection of human fibroblasts with UV-irradiated Ad5HCMVsp1lacZ. Primers flanking a 2.6-kb region of the lacZ reporter gene were added to equal amounts of DNA extracted from Ad5HCMVsp1lacZ infected cells and amplified by PCR using radiolabelled nucleotides as substrates. Results show a significant removal of UV photoproducts in normal human fibroblasts, but a reduced removal in NER deficient XP and CS cells and an enhanced removal in pre-UV-treated normal fibroblasts.

quantitative PCR host-cell reactivation adenovirus inducible DNA repair 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

LITERATURE CITED

  1. 1.
    Friedberg, E.C., Walker, G.C., and Siede, W. (1995). DNA Repair and Mutagenesis, ASMPress, Washington, DC, pp. 239–244.Google Scholar
  2. 2.
    Mellon, I., Spivak, G., and Hanawalt, P.C. (1987). Cell 51:241–249.Google Scholar
  3. 3.
    Mellon, I., Bohr, V.A., Smith, C.A., and Hanawalt, P.C. (1986). Proc. Natl. Acad. Sci. U.S.A. 83:8878–8882.Google Scholar
  4. 4.
    Balajee, A.S., and Bohr, V.A. (2000). Gene 250:15–30.Google Scholar
  5. 5.
    Cleaver, J. E. (1968). Nature 218:652–656.Google Scholar
  6. 6.
    Venema, J., van Hoffen, A., Karcagi,V., Natarajan, A.T., van Zeeland, A.A., and Mullenders, L.H. (1991). Mol. Cell. Biol. 11:4128–4134.Google Scholar
  7. 7.
    Schmickel, R.D., Chu, E.Y.H., Trosko, J.E., and Chang, C.C. (1977). Pediatrics 60:135–139.Google Scholar
  8. 8.
    Venema, J., Mullenders, L.H., Natarajan, A.T., van Zeeland, A.A., and Mayne, L.V. 1990. Proc. Natl. Acad. Sci. U.S.A. 87:4707–4711.Google Scholar
  9. 9.
    McKay, B.C., and Rainbow, A.J. (1996). Mutat. Res. 363:125–135.Google Scholar
  10. 10.
    Francis, M.A., and Rainbow, A.J. (1999). Carcinogenesis 20:19–26.Google Scholar
  11. 11.
    McKay, B.C., Francis, M.A., and Rainbow, A.J. (1997). Carcinogenesis 18:245–249.Google Scholar
  12. 12.
    Valerie, K., and Singhal, A. (1995). Mutat. Res. 336:91–100.Google Scholar
  13. 13.
    Morsy, M.A., Alford, E.L., Bett, A., Graham, F.L., and Caskey, C.T. (1993). J. Clin. Invest. 92:1580–1586.Google Scholar
  14. 14.
    Donahue, B.A., Yin, S., Taylor, J.S., Reines, D., and Hanawalt, P.C. (1994). Proc. Natl. Acad. Sci. U.S.A. 91:8502–8506.Google Scholar
  15. 15.
    Protic-Sabljic, M., and Kraemer, K.H. (1985). Proc. Natl. Acad. Sci. U.S.A. 82:6622–6626.Google Scholar
  16. 16.
    Rainbow, A.J. (1981). In Short Term Tests for Chemical Carcinogens, H.F. Stich and R.H.C. San (eds.), Springer-Verlag, New York, pp. 20–35.Google Scholar
  17. 17.
    Defais, M.J., Hanawalt, P.C., and Sarasin, A. (1983). Adv. Radiat. Biol. 10:1–37.Google Scholar
  18. 18.
    Bennett, C.B., and Rainbow, A.J. (1988). Mutagenesis 3: 157–164.Google Scholar
  19. 19.
    Jeeves, W.P., and Rainbow, A.J. (1983). Radiat. Res. 94:480–498.Google Scholar
  20. 20.
    Jeeves, W.P., and Rainbow, A.J. (1983). Int. J. Radiat. Biol. 43:599–623.Google Scholar
  21. 21.
    Jeeves, W.P., and Rainbow, A.J. (1983). Int. J. Radiat. Biol. 43:625–647.Google Scholar
  22. 22.
    Brown, T.C., and Cerutti, P.A. (1989). Mutat. Res. 2188:211–217.Google Scholar
  23. 23.
    Chakalova, L., and Russev, G. (1998). Mutat. Res. 407:147–155.Google Scholar
  24. 24.
    McCarthy, M.J., Rosenblatt, J.I., and Lloyd, R.S. (1996). Mutat. Res. 363:57–66.Google Scholar
  25. 25.
    McCarthy, M.J., Rosenblatt, J.I., and Lloyd, R.S. (1997). Photochem. Photobiol. 66:356–362.Google Scholar
  26. 26.
    Ayala-Torres, S., Chen, Y., Svoboda, T., Rosenblatt, J., and van Houten, B. (2000). Methods 22:135–147.Google Scholar
  27. 27.
    Van Houten, B., Cheng, S., and Chen, Y. (2000). Mutat. Res. 460:81–94.Google Scholar
  28. 28.
    Wellinger, R.E., and Thoma, F. (1996). Nucleic Acids Res. 24:1578–1579.Google Scholar
  29. 29.
    Graham, F.L., and Prevec, L. 1991. In Methods in Molecular Biology: Gene Transfer and Expression Protocols, Vol. 7, E.J. MurrayD, (ed.), The Humana Press, Clifton, NJ, pp. 109–128.Google Scholar
  30. 30.
    Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
  31. 31.
    Morrison, C., and Gannon, F. (1994). Biochim. Biophys. Acta 1219:493–498.Google Scholar
  32. 32.
    Rainbow, A.J., and Mak, S. (1973). Int. J. Radiat. Biol. 24:59–72.Google Scholar
  33. 33.
    Setlow, R.B., and Carrier, W.L. (1966). J. Mol. Biol. 17:237–254.Google Scholar
  34. 34.
    Protic, M., Roilides, E., Levine, A.S., and Dixon, K. (1988). Somat. Cell Mol. Genet. 14:351–357.Google Scholar
  35. 35.
    Ganesan, A.K., and Hanawalt, P.C. (1994). Somat. Cell Mol. Genet. 20:233–242.Google Scholar
  36. 36.
    Ganesan, A.K., Hunt, J., and Hanawalt, P.C. (1999). Mutat. Res. 433:117–126.Google Scholar
  37. 37.
    Barrett, S.F., Robbins, J.H., Tarone, R.E., and Kraemer, K.H. (1991). Mutat. Res. 255:281–291.Google Scholar
  38. 38.
    Lehmann, A.R., and Oomen, A. (1985). Nucleic Acids Res. 13:2087–2095.Google Scholar
  39. 39.
    Carreau, M., Eveno, E., Quilliet, X., Chevalier Lagente, O., Benoit, A., Tanganelli, B., Stefanini, M., Vermeulen, W., Hoeijmakers, J.H.J., Sarasin, A., and Mezzina, M. (1995). Carcinogenesis 16:1003–1009.Google Scholar
  40. 40.
    Klocker, H., Schneider, R., Burtscher, H.J., Auer, B., Hirsch-Kauffmann, M., and Schweiger,M. (1985). Eur. J. Cell Biol. 39:346–351.Google Scholar
  41. 41.
    Smith, M.L., Chen, I.T., Zhan, Q., O'Connor, P.M., and Fornace, A.J. (1995). Oncogene 10:1053–1059.Google Scholar
  42. 42.
    Stevsner, T., Frandsen, H., and Autrup, H. (1995). Carcinogenesis 16:2855–2858.Google Scholar
  43. 43.
    Murnane, J.P., Fuller, L.F., and Painter, R.B. (1985). Exp. Cell Res. 158:119–126.Google Scholar
  44. 44.
    Canaani, D., Naiman, T., Teitz, T., and Berg, P. (1986). Somatic Cell Mol. Genet. 12:13–20.Google Scholar
  45. 45.
    Hoeijmakers, J.H., Odijk, H., and Westerveld, A. (1987). Exp. Cell Res. 169:111–119.Google Scholar
  46. 46.
    Ford, J.M., and Hanawalt, P.C. (1995). Proc. Natl. Acad. Sci. U.S.A. 92:8876–8880.Google Scholar
  47. 47.
    Ford, J.M., and Hanawalt, P.C. (1997). J. Biol. Chem. 272:28073–28080.Google Scholar
  48. 48.
    Ford, J.M., Baron, E.L., and Hanawalt, P.C. (1998). Cancer Res. 58:599–603.Google Scholar
  49. 49.
    Therrien, J.P., Drouin, R., Baril, C., and Drobetsky, E.A. (1999). Proc. Natl. Acad. Sci. U.S.A. 96:15038–15043.Google Scholar
  50. 50.
    Squires, S., Johnson, R.T., and Collins, A.R. (1982). Mutat. Res. 95:389–404.Google Scholar
  51. 51.
    Rainbow, A.J. (1989). Carcinogenesis 10:1073–1077.Google Scholar
  52. 52.
    McKay, B.C., Winrow, C., and Rainbow, A.J. (1997). Photochem. Photobiol. 66:659–664.Google Scholar
  53. 53.
    Renzing, J., and Lane, D.P. (1995). Oncogene 10:1865–1868.Google Scholar
  54. 54.
    Siege, l.J., Fritshe, M., Mai, S., Brandner, G., and Hess, R.R. (1995). Ongogene 11:1362–1370.Google Scholar
  55. 55.
    Blagosklonny, M.V., and el-Deiry, W.S. (1996). Int. J. Cancer 67:386–392.Google Scholar
  56. 56.
    Li, G., and Ho, V.C. (1998). Br. J. Dermatol. 139:3–10.Google Scholar
  57. 57.
    Eller, M.S., Maeda, T., Magnoni, C., Atwal, D., and Gilchrest, B.A. (1997). Proc. Natl. Acad. Sci. U.S.A. 94:12627–12632.Google Scholar
  58. 58.
    Huang, J., Logsdon, N., Schmieg, F.I., and Simmons, D.T. (1998). Oncogene 17:401–411.Google Scholar
  59. 59.
    Chang, L.C., Sheu, H.M., Huang, Y.S., Tsai, T.R., and Kuo, K.W. (1999). Biochem. Pharmacol. 58:49–57.Google Scholar
  60. 60.
    Ye, N., Bianchi, M.S., Bianchi, N.O., and Holmquist, G.P. (1999). Mutat. Res. 435:43–61.Google Scholar
  61. 61.
    Germanier, M., Defais, M., Bohr, V.A., and Larminat, F. (2000). Nucleic Acids Res. 28:4674–4678.Google Scholar
  62. 62.
    McKay, B.C., Ljungman, M., and Rainbow, A.J. (1999). Carcinogenesis 20:1389–1396.Google Scholar
  63. 63.
    Smith, M.L., Chen, I.T., Zhan, Q., O'Connor, P.M., and Fornace Jr., A.J. (1995). Oncogene 10:1053–1059.Google Scholar
  64. 64.
    Smith, M.L., Kontny, H.U., Zhan, Q., Sreenath, A., O'Connor, P.M., and Fornace, A.J., Jr. (1996). Oncogene 13:2255–2263.Google Scholar
  65. 65.
    Wang, X.W., Yeh, H., Schaeffer, L., Roy, R., Moncollin, V., Egly, J.M., Wang, Z., Freidberg, E.C., Evans, M.K., Taffe, B.G., Bohr,V.A., Weeda, G., Hoeijmakers, J.H.J., Forrester, K., and Harris, C.C. (1995). Nat. Genet. 10:188–195.Google Scholar
  66. 66.
    Hwang, B.J., Ford, J.M., Hanawalt, P.C., and Chu, G. (1999). Proc. Natl. Acad. Sci. U.S.A. 96:424–428.Google Scholar
  67. 67.
    Hwang, B.J., Toering, S., Francke, U., and Chu, G. (1998). Mol. Cell. Biol.18: 4391–4399.Google Scholar
  68. 68.
    Yamaizumi, M., and Sugano, T. (1994). Oncogene 9:2775–2784.Google Scholar
  69. 69.
    Ljungman, M., and Zhang, F. (1996). Oncogene 13:823–831.Google Scholar
  70. 70.
    Ljungman, M., Zhang, F., Chen, F., Rainbow, A.J., and McKay, B.C. (1999). Oncogene 18:583–592.Google Scholar
  71. 71.
    Ko, L.J., and Prives, C. (1996). Genes Dev. 10:1054–1072.Google Scholar
  72. 72.
    McKay, B.C., Ljungman, M., and Rainbow, A.J. (1998). Oncogene 17:545–555.Google Scholar
  73. 73.
    Mayne, L., and Lehman, A. (1982). Cancer Res. 42:1473–1478.Google Scholar
  74. 74.
    Protic, M., Hirschfeld, S., Tsang, A.P., Wagner, M., Dixon, K., and Levine, A.S. 1989. Mol. Toxicol. 2:255–270.Google Scholar

Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Ihor P. Boszko
    • 1
  • Andrew J. Rainbow
    • 1
  1. 1.Department of BiologyMcMaster UniversityHamiltonCanada

Personalised recommendations