Biotechnology Letters

, Volume 24, Issue 16, pp 1379–1383

Cross-linked enzyme aggregates with enhanced activity: application to lipases

  • P. López-Serrano
  • L. Cao
  • F. van Rantwijk
  • R.A. Sheldon
Article

DOI: 10.1023/A:1019863314646

Cite this article as:
López-Serrano, P., Cao, L., van Rantwijk, F. et al. Biotechnology Letters (2002) 24: 1379. doi:10.1023/A:1019863314646

Abstract

Seven commercially available microbial lipases were immobilised as their cross-linked enzyme aggregates (CLEAs). Preparations with enhanced activity were obtained by a judicious choice of the precipitant [(NH4)2SO4, 1,2-dimethoxyethane or acetone] and by adding either a crown ether or surfactant, depending on the source of the enzyme. Thus, precipitation of the lipases from Thermomyces lanuginosus and Rhizomucor miehei with (NH4)2SO4 in the presence of SDS, followed by cross-linking with glutaraldehyde, afforded CLEAs with three and two times, respectively, the hydrolytic activity of the native enzymes. Preparations with up to ten times enhanced activity in organic medium were similarly prepared.

cross-linked enzyme aggregate hyperactivation immobilisation lipase 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • P. López-Serrano
    • 1
  • L. Cao
    • 1
  • F. van Rantwijk
    • 1
  • R.A. Sheldon
    • 1
  1. 1.Laboratory of Biocatalysis and Organic ChemistryDelftThe Netherlands

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