Biotechnology Letters

, Volume 24, Issue 17, pp 1421–1426

Ex vivo expansion of human umbilical cord blood hematopoietic progenitor cells in a novel three-dimensional culture system

  • Fuyin Xiong
  • Zhaolie Chen
  • Hong Liu
  • Zhaoping Xu
  • Xingmao Liu
Article

Abstract

A novel three-dimensional culture system for the ex vivo expansion of human umbilical cord blood (CB) hematopietic progenitor cells (HPCs) was developed by growing CB mononuclear cells on highly porous CultiSpher G microspheres coated with human bone marrow stromal cells in stirred flasks in the presence of supplemented cytokines. After 12 days, the number of total viable cells, colony-forming units in culture (CFU-C) and CD34+ cells present in the cultures reflected average increases of 7.7, 23.3 and 9.6-fold, respectively, and marked hematopoietic islands were formed on the surface of CultiSpher G.

ex vivo expansion hematopoietic progenitor cells three-dimensional culture system umbilical cord blood 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Fuyin Xiong
    • 1
  • Zhaolie Chen
    • 1
  • Hong Liu
    • 1
  • Zhaoping Xu
    • 1
  • Xingmao Liu
    • 1
  1. 1.Department of Cell EngineeringInstitute of BiotechnologyBeijingP.R. China

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