Glycoconjugate Journal

, Volume 18, Issue 7, pp 529–537

O-acetyl sialic acid specific IgM in childhood acute lymphoblastic leukaemia

  • Santanu Pal
  • Mitali Chatterjee
  • Dilip Kumar Bhattacharya
  • Santu Bandhyopadhyay
  • Chhabinath Mandal
  • Chitra Mandal
Article

Abstract

Initial studies have revealed an enhanced surface expression of O-acetylated sialoglycoconjugates (O-AcSGs) on lymphoblasts concomitant with high titres of IgG in childhood Acute Lymphoblastic Leukaemia (ALL) (Mandal C, Chatterjee M, Sinha D, Br J Haematol 110, 801–12, 2000). In our efforts to identify disease specific markers for ALL, we have affinity-purified IgM directed against O-AcSGs that reacts with three disease specific O-AcSGs present on membrane proteins derived from peripheral blood mononuclear cells (PBMC) of ALL patients. Antibody specificity towards O-AcSGs was confirmed by selective binding to erythrocytes bearing surface O-AcSGs, decreased binding with de-O-acetylated BSM and following pretreatment with O-acetyl esterase. Competitive inhibition ELISA demonstrated a higher avidity of IgM for O-AcSG than IgG. Flow cytometry demonstrated the diagnostic potential of purified O-AcSA IgM as binding was specific with ALL patients and minimal with other haematological disorders and normal individuals. It therefore may be adopted as a non-invasive approach for detection of childhood ALL. Taken together, the data indicates that carbohydrate epitopes having terminal O-AcSA α2 → 6 GalNAc determinants induce disease specific IgG and IgM, potentially useful molecular markers for childhood ALL.

acute lymphoblastic leukaemia (ALL) O-acetylated sialic acids minimal residual disease IgM antibodies against O-acetylated sialic acids 

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References

  1. 1.
    Mandal C, Chatterjee M, Sinha D, Investigation of 9-Oacetylated sialoglycoconjugates in childhood acute Lymphoblastic leukaemia, Br J Haematol 110, 801–12 (2000).Google Scholar
  2. 2.
    Kelm S, Schauer R, Sialic acids in molecular and cellular interactions, Int Rev Cytol 175, 137–240 (1997).Google Scholar
  3. 3.
    Schauer R, Chemistry metabolism and biological function of sialic acids, Adv Carb Chem Biochem 40, 131–234 (1982).Google Scholar
  4. 4.
    Varki A, Diversity in sialic acids, Glycobiology 2, 25–40 (1992).Google Scholar
  5. 5.
    Kamerling JP, Makovitzky J, Schauer R, Vliegenthart JF, Wember M, The nature of sialic acids in human lymphocytes, Biochim Biophys Acta 714, 351–5 (1982).Google Scholar
  6. 6.
    Schwartz-Albiez R, Carbohydrate and Lectin Section Leucocyte Typing VII, edited by Mason D, et al. (Oxford University Press, 2001), pp. 149–64.Google Scholar
  7. 7.
    Mandal C, Basu S, A unique specificity of a sialic acid binding lectin AchatininH from the hemolymph of Achatina fulica snail, Biochem Biophys Res Comm 148, 795–801 (1987).Google Scholar
  8. 8.
    Mandal C, Basu S, Mandal C, Physicochemical studies on achatininH a novel sialic acid binding lectin, Biochem J 257, 65–71 (1989).Google Scholar
  9. 9.
    Sen G, Mandal C, The specificity of the binding site of AchatininH a sialic-acid binding lectin from Achatina fulica, Carbohydr Res 268, 115–25 (1995).Google Scholar
  10. 10.
    Sen G, Chowdhury M, Mandal C, O-acetylated sialic acid as a distinct marker for differentiation between several leukemia erythrocytes, Mol Cell Biochem 136, 65–70 (1994).Google Scholar
  11. 11.
    Sharma V, Chatterjee M, Mandal C, Sen S, Basu D, Rapid diagnosis of Indian visceral leishmaniasis using achatininH, a 9-Oacetylated sialic acid binding lectin, Am J Trop Med Hyg 58, 551–4 (1998).Google Scholar
  12. 12.
    Sinha D, Mandal C, Bhattacharya DK, Identification of 9-O-acetyl sialoglycoconjugates (9-O-AcSGs) as biomarkers in childhood acute lymphoblastic leukemia using a lectin, Achatinin-H, as a probe, Leukemia 13, 119–25 (1999a).Google Scholar
  13. 13.
    Pal S, Chatterjee M, Bhattacharya DK, Bandhyopadhyay S, Mandal C, Identification and purification of cytolytic antibodies directed against O-acetylated sialic acid in childhood acute lymphoblastic leukemia, Glycobiology 10, 539–49 (2000).Google Scholar
  14. 14.
    Chatterjee M, Sharm V, Sundar S, Sen S, Mandal C, Identification of antibodies directed against O-acetylated sialic acids in visceral leishmaniasis, its diagnostic and prognostic role, Glycoconjugate J 15, 1141–7 (1998).Google Scholar
  15. 15.
    Sinha D, Chatterjee M, Mandal C, O-acetylated sialic acids-their detection biological significance and alteration in diseases, TIGG 12, 17–33 (2000).Google Scholar
  16. 16.
    Harris NL, Jaffe ES, Stein H, Banks PM, Chan JK, Cleary ML, Delsol G, De Wolf-Peeters C, Falini B, Gatter KC, A revised European-American classification of lymphoid neoplasms: A proposal from the International Lymphoma study group, Blood 84, 1361–92 (1994).Google Scholar
  17. 17.
    Siebert H-C, von der Lieth C-W, Dong X, Reuter G, Schauer R, Gabius H-J, Vliegenthart JF, Molecular dynamics-derived conformation and intramolecular interaction analysis of the N-acetyl-9-O-acetylneuraminic acid-containing gangliosideGD1a and NMR-based analysis of its binding to a human polyclonal immunoglobulin G fraction with selectivity for O-acetylated sialic acids, Glycobiology 6, 561–72 (1996).Google Scholar
  18. 18.
    Coligan EJ, Kruisbeek MA, Margulies HD, Shevach ME, Strober W, Current protocols in Immunology National Institute of Health 1 5.3.2–4 (1993).Google Scholar
  19. 19.
    Reuter G, Pfeil R, Stoll S, Schauer R, Kamerling JP, Versluis C, Vliegenthart JF, Identification of new sialic acids derived from glycoprotein of bovine submandibular gland, Eur J Biochem 134, 139–43 (1983).Google Scholar
  20. 20.
    Zimmer G, Reuter G, Schauer R, Use of influenza C virus for detection of 9-O-acetylated sialic acids on immobilized glycoconjugates by esterase activity, Eur J Biochem 204, 209–15 (1992).Google Scholar
  21. 21.
    Wu X, Okada N, Momota H, Irie RF, Okada H, Complementmediated anti-HIV-1 effect induced by human IgM monoclonal antibody against ganglioside GM2, J Immunol 162, 533–9 (1999).Google Scholar
  22. 22.
    Neale GA, Coustan-Smith E, Pan Q, Chen X, Gruhn B, Stow P, Behm FG, Pui CH, Campana D, Tandem application of flow cytometry and polymerase chain reaction for comprehensive detection of minimal residual disease in childhood acute lymphoblastic leukemia, Leukemia 13, 1221–6 (1999).Google Scholar
  23. 23.
    Sharma V, Chatterjee M, Sen G, Ch. Anil Kumar, Mandal C, Role of linkage specific 9-O-acetylated sialoglycoconjugates in activation of the alternate complement pathway in mammalian erythrocytes, Glycoconjugate J 17, 887–93 (2000).Google Scholar
  24. 24.
    Pui C-H, Childhood leukemias, N Engl J Med 332, 1618–30 (1995).Google Scholar
  25. 25.
    Chen J-S, Coustan-Smith E, Suzuki T, Neale GA, Mihara K, Pui C-H, Campana D, Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia. Blood 97, 2115–20 (2001).Google Scholar
  26. 26.
    Campana D, Neale GA, Coustan-Smith E, Pui CH, Detection of minimal residual disease in acute lymphoblastic leukemia, the St Jude experience, Leukemia 15, 278–9 (2001).Google Scholar
  27. 27.
    Sinha D, Mandal C, Bhattacharya DK, A novel method for prognostic evaluation of childhood acute lymphoblastic leukemia, Leukemia 13, 309–12 (1999b).Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Santanu Pal
    • 1
  • Mitali Chatterjee
    • 1
  • Dilip Kumar Bhattacharya
    • 2
  • Santu Bandhyopadhyay
    • 3
  • Chhabinath Mandal
    • 4
  • Chitra Mandal
    • 5
  1. 1.Immunobiology DivisionIndian Institute of Chemical BiologyCalcuttaIndia
  2. 2.Vivekananda Institute of Medical SciencesCalcuttaIndia
  3. 3.Cellular ImmunologyIndian Institute of Chemical BiologyCalcuttaIndia
  4. 4.Protein Design and Engineering DivisionsIndian Institute of Chemical BiologyCalcuttaIndia
  5. 5.Immunobiology DivisionIndian Institute of Chemical BiologyCalcuttaIndia

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