Cell Biology and Toxicology

, Volume 18, Issue 5, pp 321–328 | Cite as

Immortalized renal proximal and collecting duct cell lines derived from transgenic mice harboring L-type pyruvate kinase promoters as tools for pharmacological and toxicological studies

  • A. Vandewalle


Targeted oncogenesis in transgenic mice, where an oncogene is placed under the control of the regulatory sequences of a cell-specific gene, has been used to derive lines of differentiated kidney epithelial cells derived from proximal or distal tubules or from the collecting duct. These renal cell lines were obtained from kidneys of transgenic mice harboring the large-T and little-t antigens placed under the control of regulatory sequences of the L-type pyruvate kinase gene. This review summarizes the main properties of these differentiated cell lines, which are usefulex vivo cell systems for pharmacological and toxicological studies.

kidney transgenic mice cell culture proximal cells collecting duct cells 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • A. Vandewalle
    • 1
  1. 1.INSERM U478, Faculté de Médecine Xavier BichatInstitut National de la Santé et de la Recherche MédicaleParisFrance

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