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Cell Biology and Toxicology

, Volume 18, Issue 5, pp 321–328 | Cite as

Immortalized renal proximal and collecting duct cell lines derived from transgenic mice harboring L-type pyruvate kinase promoters as tools for pharmacological and toxicological studies

  • A. Vandewalle
Article

Abstract

Targeted oncogenesis in transgenic mice, where an oncogene is placed under the control of the regulatory sequences of a cell-specific gene, has been used to derive lines of differentiated kidney epithelial cells derived from proximal or distal tubules or from the collecting duct. These renal cell lines were obtained from kidneys of transgenic mice harboring the large-T and little-t antigens placed under the control of regulatory sequences of the L-type pyruvate kinase gene. This review summarizes the main properties of these differentiated cell lines, which are usefulex vivo cell systems for pharmacological and toxicological studies.

kidney transgenic mice cell culture proximal cells collecting duct cells 

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References

  1. Altan N, Chen Y, Schindler M, Simon SM. Defective aci¢ca-tion in human breast tumor cells and implications for chemotherapy. J Exp Med. 1998; 187: 1583–98.PubMedCrossRefGoogle Scholar
  2. Bens M, Bogdanova A, Cluzeaud F, et al. Transimmortalized mouse intestinal cells (m-ICcl2) that maintain a crypt phenotype. Am J Physiol. 1996; 270: C1666–74.PubMedGoogle Scholar
  3. Bens M, Vallet V, Cluzeaud F, et al. Corticosteroid-dependent sodium transport in a novel immortalized mouse collecting duct principal cell line. J Am Soc Nephrol. 1999; 10: 923–34.PubMedGoogle Scholar
  4. Briand P, Kahn A, Vandewalle A. Targeted oncogenesis: a powerful method to derive renal cell lines. Kidney Int. 1995; 47: 388–94.PubMedGoogle Scholar
  5. Canessa CM, Schild L, Buell G, et al. The amiloride-sensitive epithelial sodium channel is made of three homologous subunits. Nature. 1994; 367: 463–7.PubMedCrossRefGoogle Scholar
  6. Cartier N, Miquerol L, Tulliez M, et al. Diet-dependent carcinogenesis of pancreatic islets and liver in transgenic mice expressingoncogenes under the control of the L-type pyruvate kinase gene promoter. Oncogene. 1992; 7: 1413–22.PubMedGoogle Scholar
  7. Cartier N, Lacave R, Vallet V et al. Establishment of renal proximal tubule cell lines by targeted oncogenesis in transgenic mice using the L-pyruvate kinase-SV40 (T) antigen hybrid gene. J Cell Sci. 1993; 104: 695–704.PubMedGoogle Scholar
  8. Cebrian C, Areste C, Nicolas A, et al. Kidney androgen-regulated protein interacts with cylcophilin B and reduces cyclosporine A-mediated toxicity in proximal tubule cells. J Biol Chem. 2001; 276: 29410–9.PubMedCrossRefGoogle Scholar
  9. Cognet M, Lone YC, Vaulont S, Kahn A, Marie J. Structure of the rat L-type pyruvate kinase gene. J Mol Biol. 1987; 196: 11–25.PubMedCrossRefGoogle Scholar
  10. Compere SJ, Baldacci P, Jaenisch R. Oncogenes in transgenic mice. Biochim Biophys Acta. 1988; 948: 129–49.PubMedGoogle Scholar
  11. Courjault-Gautier F, Antoine B, Bens M, et al. Activity and inducibility of drug-metabolizing enzymes in immortalized hepatocyte-like cells (mhPKT) derived of a L-PK/Tag1 transgenic mouse. Exp Cell Res. 1997; 234: 362–72.PubMedCrossRefGoogle Scholar
  12. Decaux JF, Antoine B, Kahn A. Regulation of the expression of the L-type pyruvate kinase gene in adult hepatocytes in primary culture. J Biol Chem. 1989; 264: 11584–90.PubMedGoogle Scholar
  13. Duc C, Farman N, Canessa CM, Bonvalet JP, Rossier BC. Cell-speci¢c expression of epithelial sodium channel a, b, g subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry. J Cell Biol. 1994; 127: 1907–21.PubMedCrossRefGoogle Scholar
  14. DuongVan Huyen JP, Bens M, Cluzeaud F, Vandewalle A. Differential effects aldosterone and vasopressin on chloride £uxes in transimmortalized mouse cortical collectingducts cells. J Membr Biol. 1998; 164: 79–90.CrossRefGoogle Scholar
  15. DuongVan Huyen JP, Bens M, Teulon J, Vandewalle A. Vasopressin-stimulated chloride transport in transimmortalized mouse cell lines derived from the distal convoluted tubule and cortical and inner medullary collectingducts. Nephrol Dial Transplant. 2001; 16: 238–45.CrossRefGoogle Scholar
  16. Fanning E, Knippers R. Sructure and function of simian virus 40 large tumor antigen. Annu Rev Biochem 1992; 61: 55–85.PubMedCrossRefGoogle Scholar
  17. Gstraunthaler G, Pfaller W, Kotento P. Biochemical characterization of renal epithelial cell cultures (LLC-PK1 and MDCK). Am J Physiol. 1985; 248: F536–44.PubMedGoogle Scholar
  18. Hanahan D. Dissectingmultistep tumorigenesis in transgenic mice. Annu Rev Genet. 1988; 22: 479–519.PubMedCrossRefGoogle Scholar
  19. Hunter SE, Clarke IN, Kelly DC, Titball EW. Cloningand nucleotide sequencingof the Clostridium perfringens epsi-lon-toxin gene and its expression in Escherichia coli. Infect Immun. 1992; 60: 102–10.PubMedGoogle Scholar
  20. Imamura K, Tanaka T. Pyruvate kinase isoenzymes from rat. Methods Enzymol. 1982; 90: 150–6.PubMedCrossRefGoogle Scholar
  21. Kamynina E, Debonneville C, Bens M, Vandewalle A, Staub O. A novel Nedd4 protein supresses the activity of the epithelial Na+ channel. FASEB J. 2001; 15: 204–14.PubMedCrossRefGoogle Scholar
  22. Lacave R, Bens M, Cartier N, et al. Functional characteristics of proximal tubule cell lines derived from transgenic mice harboring L-pyruvate kinase-SV40 (T) antigen hybrid gene. J Cell Sci. 1993; 104: 705–12.PubMedGoogle Scholar
  23. Lacave R, Ouar Z, Paulais M, et al. Lysosomotropic agents increase vinblastine e¥ux from mouse MDR proximal kidney cells exhibitingvectorial drugtransport. J Cell Physiol. 1999; 178: 247–57.PubMedCrossRefGoogle Scholar
  24. Livingston D, Bradley P. The simian virus 40 large T antigen: a lot packed into a little. Mol Biol Med. 1987; 4: 63–80.PubMedGoogle Scholar
  25. McDonnell JL. Toxins of Clostridium perfringens type A, B, C, D and E. In: Dorner F and Drews J, eds. Pharmacology of bacterial toxins. Oxford: Pergamon Press; 1986: 477–517.Google Scholar
  26. Miquerol L, Cluzeaud F, Porteu A, Alexandre Y, Vandewalle A, Kahn, A. Tissue speci¢city of L-pyruvate kinase transgenes results from combinatorial effects of proximal promoter and distal activator regions. Gene Expression. 1996; 5: 315–30.PubMedGoogle Scholar
  27. Munnich A, Marie J, Reach G, Vaulont S, Simon MP, Kahn A. In vivo hormonal control of L-type pyruvate kinase gene expression. J Biol Chem. 1984; 259: 10228–31.PubMedGoogle Scholar
  28. Ouar Z, Sole¨ E, Bens M, Rafestin-Oblin M.E, Meseguer A, Vandewalle A. Pleiotropic effects of dihydrotestosterone in immortalized mouse proximal cells. Kidney Int. 1998; 53: 59–66.PubMedCrossRefGoogle Scholar
  29. Ouar Z, Lacave R, Bens M, Vandewalle A. Mechanisms of altered sequestration and e¥ux of chemotherapeutic drugs by multidrugresistant cells. Cell Biol Toxicol. 1999; 15: 91–100.PubMedCrossRefGoogle Scholar
  30. Petit L, Gibert M, Gillet M, Laurent-Winter C, Boquet P, Popoff MR. Clostridium perfringens epsilon-toxin acts on MDCK cells by forminglarge membrane complex. J Bacteriology. 1997; 179: 6480–7.Google Scholar
  31. Riccaldi D, Robic D, Bens M, et al. Cultured proximal cells derived from transgenic mouse provide model to study drug toxicity. Kidney Int. 1995; 48: 722–30.PubMedGoogle Scholar
  32. Robert-Nicoud M, Flahaut M, Elalouf JM, et al. Transcriptome of a mouse cortical collectingduct cell line: effects of aldosterone and vasopressin. Proc Natl Acad Sci USA. 2001; 98: 2712–6.PubMedCrossRefGoogle Scholar
  33. Rossier BC, Palmer LG. The kidney: mechanisms of aldosterone action on sodium and potassium transport. In: Seldin DW and Giebiesch G, eds. The kidney: physiology and pathophysiology. New York: Raven Press; 1992: 1373–409.Google Scholar
  34. Schindler M, Grabski S, Hoof E, Simon SM. Defective pH regulation of acidic compartments in human breast cancer cells (MCF-7) is normalized in adriamycin-resistant cells (MCF-7adr). Biochemistry. 1996; 35: 2811–7.PubMedCrossRefGoogle Scholar
  35. Simon M, Schindler M. Cell biological mechanisms of multidrugresistance in tumors. Proc Natl Acad Sci USA. 1994; 91: 3497–504.PubMedCrossRefGoogle Scholar
  36. Staub O, Dho S, Henry P, et al. WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+ channel deleted in Liddle's syndrome. EMBO J. 1996; 15: 2371–80.PubMedGoogle Scholar
  37. Vallet V, Bens M, Antoine B, et al. Transcription factors and aldolase B gene expression in microdissected tubules and derived cell lines. Exp Cell Res. 1995; 216: 363–70.PubMedCrossRefGoogle Scholar
  38. Vandewalle A. Stimulated secretion of lysosomal enzymes induced by drugs in transimmortalized proximal tubule mouse kidney cells. Cell Biol Toxicol. 1996; 12: 299–303.PubMedCrossRefGoogle Scholar
  39. Vaulont S, Munnich A, Decaux JF, Kahn A. Transcriptional and post-transcriptional regulation of L-type pyruvate kinase gene expression in rat liver. J Biol Chem. 1986; 261: 7621–5.PubMedGoogle Scholar
  40. Voisin T, Bens M, Cluzeaud F, Vandewalle A, Laburthe M. Peptide YY receptors in the proximal tubule PKSV-PCT cell line derived from transgenic mice – relation with cell growth. J Biol Chem. 1993; 268: 20547–54.PubMedGoogle Scholar
  41. Voisin T, Lorinet AM, Maoret JJ, Couvineau A, Laburthe M. Gai RNA antisense expression demonstrates the exclusive couplingof peptide YY receptors to Gi2 proteins in renal proximal tubule cells. J Biol Chem. 1996; 271: 574–80.PubMedCrossRefGoogle Scholar
  42. Vuagniaux G, Vallet V, Fowler Jeager N, et al. Activation of the amiloride-sensitive epithelial sodium channel by the mouse serine protease mCAP1 expressed in a mouse cortical collectingduct cell line. J Am Soc Nephrol. 2000; 11: 828–34.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • A. Vandewalle
    • 1
  1. 1.INSERM U478, Faculté de Médecine Xavier BichatInstitut National de la Santé et de la Recherche MédicaleParisFrance

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