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Pharmaceutical Research

, Volume 9, Issue 2, pp 178–181 | Cite as

Potentiation of Cytotoxicity of Mitoxantrone Toward CHO-K1 Cells in Vitro by Dipyridamole

  • Pankaj B. Desai
  • Rajagopalan Sridhar
Article

Abstract

Dipyridamole (DP), a clinically used vasodilator and an antiplatelet compound, augmented the activity of the anticancer drug mitoxantrone (MXN) toward Chinese hamster ovary (CHO-K1) cells in culture. Clonogenic assays indicated that DP (1.0, 2.5, and 5.0 µM) decreased the survival of cells treated with MXN (5 to 25 nM) in a dose-dependent manner. Further, DP (1 and 5 nM) decreased the MXN concentration required for 50% inhibition of cell growth from 3.2 to 1.8 and from 3.0 to 0.5 nM, respectively, over a period of 3 days. DP (10 µM) increased the accumulation of MXN by 1.8-fold in exponentially growing cells exposed to MXN. The enhanced levels of MXN in CHO-K1 cells in the presence of the chemosensitizer may account for the potentiation of MXN-cytotoxicity by DP.

mitoxantrone cytotoxicity chemosensitization CHO-K1 cells dipyridamole 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Pankaj B. Desai
    • 1
  • Rajagopalan Sridhar
    • 1
    • 2
  1. 1.Department of Basic Pharmaceutical Sciences, College of PharmacyUniversity of South CarolinaColumbia
  2. 2.Department of Radiation Therapy and Cancer CenterHoward UniversityWashington

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