Pharmaceutical Research

, Volume 16, Issue 2, pp 255–260 | Cite as

A Polysorbate-Based Non-Ionic Surfactant Can Modulate Loading and Release of β-Lactoglobulin Entrapped in Multiphase Poly(DL-Lactide-co-glycolide) Microspheres

  • Judith Rojas
  • Huguette Pinto-Aiphandary
  • Eliana Leo
  • Sophie Pecquet
  • Patrick Couvreur
  • Annette Gulik
  • Elias Fattal


Purpose. The goal of the present paper was to investigate the role of a surfactant, Tween 20, in the modulation of the entrapment and release of β-lactoglobulin (BLG) from poly (DL-lactide-co-glycolide) microspheres.

Methods. Poly(DL-lactide-co-glycolide) microspheres containing BLG were prepared by a water-in-oil-in-water emulsion solvent procedure. Tween 20 was used as a surfactant in the internal aqueous phase of the primary emulsion. BLG entrapment efficiency and burst release were determined. Displacement of BLG from microsphere surface was followed by confocal microscopy observations and zeta potential measurements, whereas morphological changes were observed by freeze-fracture electron microscopy.

Results. Tween 20 was shown to increase 2.8 fold the encapsulation efficiency of BLG without any modification of the stability of the first emulsion and the viscosity of the internal aqueous phase. In fact, Tween 20 was shown to be responsible for removing the BLG molecules that were adsorbed on the particle surface or very close to the surface as shown by confocal microscopy and zeta potential measurements. Tween 20 reduced the number of aqueous channels between the internal aqueous droplets as well as those communications with the external medium. Thus, the more dense structure of BLG microspheres could explain the decrease of the burst release.

Conclusions. These results constitute a step forward in the improvement of existing technology in controlling protein encapsulation and delivery from microspheres prepared by the multiple emulsion solvent evaporation method.

biodegradable microspheres poly(DL-lactide-co-gly-colide) (β-lactoglobulin Tween 20 encapsulation protein aggregation 


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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Judith Rojas
    • 1
  • Huguette Pinto-Aiphandary
    • 1
  • Eliana Leo
    • 2
  • Sophie Pecquet
    • 3
  • Patrick Couvreur
    • 1
  • Annette Gulik
    • 4
  • Elias Fattal
    • 1
  1. 1.School of Pharmacy, URA CNRS 1218University of Paris-SudChâtenay-MalabryFrance
  2. 2.Department of Pharmaceutical SciencesUniversity of ModenaModenaItaly
  3. 3.Nestlé SA, Nestle Research Center LausanneLausanne 26Switzerland
  4. 4.Centre de Génétique Moléculaire, UPR A2420Gif-sur-Yvette CedexFrance

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