Digestive Diseases and Sciences

, Volume 43, Issue 1, pp 162–165

Colonic Luminal Hydrogen Sulfide Is Not Elevated in Ulcerative Colitis

  • J. Moore
  • W. Babidge
  • S. Millard
  • W. Roediger


It has been proposed that the reduction inn-butyrate oxidation by colonic epithelial cellsobserved in ulcerative colitis may be related toexposure to reduced forms of sulfur derived fromdissimilatory sulfate reduction by luminal microflora. Thisstudy aims to compare stool sulfide concentrations incontrol and colitic subjects. Control subjects hadsignificant colorectal disease excluded by virtue of their selection. Patients with ulcerativecolitis were stratified by disease extent and activity,and by salicylate drug use. Stool sulfide was measuredusing a direct spectrophotometric method on NaOH (free sulfide) and zinc acetate (total sulfide)stool slurries. Fifteen control and 19 colitic subjectswere studied. There was no significant difference instool sulfide between control and colitic patients (free sulfide, control =0.52 (0.17), colitic0.45 (0.10), t = 0.36, P = 0.71, total sulfide, control1.33 (0.21), colitic 0.96 (0.15), t = 1.44, P = 0.16).Disease extent or activity did not significantlyinfluence stool sulfide. These results do not support aprimary etiologic role for luminal sulfide in ulcerativecolitis.



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  1. 1.
    Roediger WEW, Duncan A, Kapaniris O, Millard S: Sulfide impairment of substrate oxidation in rat colonocyte s: A biochemical basis for ulcerative colitis. Clin Sci 85:623–627, 1993Google Scholar
  2. 2.
    Roediger WEW, Duncan A, Kapaniris O, Millard S: Reducing sulphur compounds of the colon impair colonocyte nutrition: Implications for ulcerative colitis. Gastroenterology 104:802–809, 1993Google Scholar
  3. 3.
    Florin THJ, Khalil D, Lenarczyk A, Cowley DM: Sulfur me tabolism in colons of carrageenan fed rats. J Gastroenterol Hepatol 10:A56, 1995Google Scholar
  4. 4.
    Gibson GR, Cummings JH, MacFarlane GT: Growth and ctivities of sulfate reducing bacteria in gut contents of healthy subjects and patients with ulcerative colitis. FEMS Microbiol Ecol 86:103–112, 1991Google Scholar
  5. 5.
    Pitcher MCL, Beatty ER, Gibson GR, Cummings JH: Incidence and activities of sulfate reducing bacteria in patients with ulcerative colitis. Gut 36(suppl 1):A63, 1995Google Scholar
  6. 6.
    Florin THJ, Gibson GR, Neale G, Cummings JH: A role for sulphate reducing bacteria in ulcerative colitis? Gastroenterology 98:A170, 1990Google Scholar
  7. 7.
    McIntyre PB, Macrae FA, Berghouse L, English J, Lennard-Jones JE: Therapeutic bene fits from a poorly absorbed prednisolone enema in distal colitis. Gut 26:822–824, 1985Google Scholar
  8. 8.
    Florin THJ: Hydroge n sulfide and total acid volatile sulfide in faeces, determined with a direct spectrophotometric method. Clin Chim Acta 196:127–134, 1991Google Scholar
  9. 9.
    Chapman MAS, Grahn M, Boyle M, Hutton M, Rogers J, Williams NS: Butyrate metabolism is impaired in the colonic mucosa of suffere rs of quiescent ulcerative colitis. Gut 35:73–76, 1994Google Scholar
  10. 10.
    Roediger WEW: The colonic epithelium in ulcerative colitis: an energy deficient disease? Lancet 2:712–715, 1980Google Scholar
  11. 11.
    Pitcher MCL, Beatty ER, Cummings JH: Salicylates inhibit bacterial sulfide production within the colonic lumen in ulcerative colitis. Gut 37( suppl 2):A15, 1995Google Scholar

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • J. Moore
  • W. Babidge
  • S. Millard
  • W. Roediger

There are no affiliations available

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