Pharmaceutical Research

, Volume 16, Issue 4, pp 514–518

Cell Loading with Laser-Generated Stress Waves: The Role of the Stress Gradient

  • Stephen E. Mulholland
  • Shun Lee
  • Daniel J. McAuliffe
  • Apostolos G. Doukas
Article

DOI: 10.1023/A:1018814911497

Cite this article as:
Mulholland, S.E., Lee, S., McAuliffe, D.J. et al. Pharm Res (1999) 16: 514. doi:10.1023/A:1018814911497

Abstract

Purpose. To determine the dependence of the permeabilzation of the plasma membrane on the characteristics of laser-generated stress waves.

Methods. Laser pulses can generate stress waves by ablation. Depending on the laser wavelength, fluence, and target material, stress waves of different characteristics (rise time, peak stress) can be generated. Human red blood cells were subjected to stress waves and the permeability changes were measured by uptake of extracellular dye molecules.

Results. A fast rise time (high stress gradient) of the stress wave was required for the permeabilization of the plasma membrane. While the membrane was permeable, the cells could rapidly uptake molecules from the surrounding medium by diffusion.

Conclusions. Stress waves provide a potentially powerful tool for drug delivery.

drug delivery drug carriers red blood cells sono-phoresis shock waves ultrasound phonophoresis phonoporation 

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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Stephen E. Mulholland
    • 1
  • Shun Lee
    • 1
  • Daniel J. McAuliffe
    • 1
  • Apostolos G. Doukas
    • 1
  1. 1.Department of Dermatology, Harvard Medical SchoolWellman Laboratories of Photomedicine, Massachusetts General HospitalBoston
  2. 2.Genetics InstituteAndover

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