Glycoconjugate Journal

, Volume 14, Issue 4, pp 449–456

Isolation, characterization and molecular cloning of the bark lectins from Maackia amurensis

  • Els J.M Van Damme
  • Fred Van Leuven
  • Willy J Peumans


A detailed study was made of the bark lectins of the legume tree Maackia amurensis using a combination of protein purification and cDNA cloning. The lectins, which are the most abundant bark proteins, are a complex mixture of isoforms composed of two types of subunits of 32 and 37 kDa, respectively. Isolation and characterization of the homotetrameric isoforms indicated that the 32 kDa subunit exhibits a 100-fold stronger haemagglutinating activity than the 37 kDa subunit. Molecular cloning confirmed that the two lectin subunits are encoded by different genes. The 32 kDa subunit is apparently encoded by a single gene, whereas two highly homologous genes encode the 37 kDa subunit. A comparison of the deduced amino acid sequences of the bark lectin cDNAs and the previously described cDNA encoding the seed haemagglutinin demonstrated that they are encoded by different genes. Abbreviations: LECMAHb, cDNA clone encoding Maackia amurensis bark haemagglutinin; LECMALb, cDNA clone encoding Maackia amurensis bark leucoagglutinin; MALb, Maackia amurensis bark leucoagglutinin; MAHb, Maackia amurensis bark haemagglutinin

bark proteins Maackia amurensis cDNA cloning lectin 


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  1. 1.
    Sharon N, Lis H (1990) FASEB J 4: 3198-208.PubMedGoogle Scholar
  2. 2.
    Sata T, Lackie PM, Taatjes J, Peumans W, Roth J (1989) J Histochem Cytochem 37: 1577-88.PubMedGoogle Scholar
  3. 3.
    Kawaguchi T, Matsumoto I, Osawa T (1974) J Biol Chem 249: 2786-92.PubMedGoogle Scholar
  4. 4.
    Wang W-C, Cummings RD (1988) J Biol Chem 263: 4576-85.PubMedGoogle Scholar
  5. 5.
    Knibbs R, Goldstein IJ, Ratcliff RM, Shibuya N (1991) J Biol Chem 266: 83-88.PubMedGoogle Scholar
  6. 6.
    Konami Y, Yamamoto K, Osawa T, Irimura T (1994) FEBS Lett 342: 334-38.PubMedCrossRefGoogle Scholar
  7. 7.
    Konami Y, Ishida C, Yamamoto K, Osawa T, Irimura T (1994) J Biochem 115: 767-77.PubMedGoogle Scholar
  8. 8.
    Yamamoto K, Ishida C, Saito M, Konami Y, Osawa T, Irimura T (1994) Glycoconjugate J 11: 572-75.CrossRefGoogle Scholar
  9. 9.
    Laemmli UK (1970) Nature 227: 680-85.PubMedCrossRefGoogle Scholar
  10. 10.
    Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Anal Chem 28: 350-56.CrossRefGoogle Scholar
  11. 11.
    Van Damme EJM, Peumans WJ (1993) In Lectins and Glycobiology (Gabius H-J, Gabius S, eds) pp. 458-68, Germany: Springer Verlag.Google Scholar
  12. 12.
    Van Damme EJM, Barre A, Bemer V, Rougé P, Van Leuven F, Peumans WJ (1995) Plant Mol Biol 29: 579-98.PubMedCrossRefGoogle Scholar
  13. 13.
    Van Damme EJM, Barre A, Smeets K, Torrekens S, Van Leuven F, Rougé P, Peumans WJ (1995) Plant Physiol 107: 833-43.PubMedCrossRefGoogle Scholar
  14. 14.
    Van Damme EJM, Goldstein IJ, Vercammen G, Vuylsteke J, Peumans WJ (1992) Physiol Plant 86: 245-52.CrossRefGoogle Scholar
  15. 15.
    Mierendorf RC, Pfeffer D (1987) Meth Enzymol 152: 556-62.PubMedCrossRefGoogle Scholar
  16. 16.
    Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74: 5463-67.PubMedCrossRefGoogle Scholar
  17. 17.
    Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York, USA: Cold Spring Harbor Laboratory.Google Scholar
  18. 18.
    Van Damme EJM, Smeets K, Torrekens S, Van Leuven F, Goldstein IJ, Peumans WJ (1992) Eur J Biochem 206: 413-20. PubMedCrossRefGoogle Scholar
  19. 19.
    de Kochko A, Hamon S (1990) Plant Mol Biol Rep 8: 3-7.Google Scholar
  20. 20.
    Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol Biol Rep 1: 19-21.Google Scholar
  21. 21.
    Feldsted RL, Egorin MJ, Leavitt RD, Bachur NR (1977) J Biol Chem 252: 2967-71.Google Scholar
  22. 22.
    von Heijne G (1986) Nucleic Acids Res 11: 4683-90.Google Scholar
  23. 23.
    Van Damme EJM, Barre A, Rougé P, Van Leuven F, Peumans WJ (1995) Plant Mol Biol 29: 1197-210.PubMedCrossRefGoogle Scholar
  24. 24.
    Van Damme EJM, Barre A, Rougé P, Peumans WJ (1997) Plant Mol Biol 33: 523-36.PubMedCrossRefGoogle Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Els J.M Van Damme
    • 1
  • Fred Van Leuven
    • 2
  • Willy J Peumans
    • 1
  1. 1.Laboratory for Phytopathology and Plant ProtectionKatholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Center for Human GeneticsKatholieke Universiteit LeuvenLeuvenBelgium

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