Glycoconjugate Journal

, Volume 14, Issue 1, pp 75–80

Enzymatic syntheses of GlcNAcβ1-2Man and Galβ1-4GlcNAcβ1-2Man as components of complex type sugar chains

  • Hiroshi Fujimoto
  • Megumi Isomura
  • Tatsuo Miyazaki
  • Ichiro Matsuo
  • Richard Walton
  • Tohru Sakakibara
  • Katsumi Ajisaka
Article

Abstract

GlcNAcβ1-2Man and GlcNAcβ1-6Man were synthesized using the reverse hydrolysis activity of β-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Galβ1-4GlcNAcβ1-2Man and Galβ1-4GlcNAcβ1-6Man were synthesized regioselectively using the transglycosylation activity of β-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies. Abbreviations: pNp-β-GlcNAc, p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; pNp-β-Gal, p-nitrophenyl β-D-galacto-pyranoside

GlcNAcβ1-2Man Galβ1-4GlcNAcβ1-2Man Galβ1-4GlcNAcβ1-6Man components of complex type sugar chains 

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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Hiroshi Fujimoto
    • 1
  • Megumi Isomura
    • 1
  • Tatsuo Miyazaki
    • 2
  • Ichiro Matsuo
    • 1
  • Richard Walton
    • 1
  • Tohru Sakakibara
    • 2
  • Katsumi Ajisaka
    • 1
  1. 1.Meiji Institute of Health Science NarudaOdawaraJapan
  2. 2.Faculty of Science, Department of ChemistryYokohama City UniversityYokohamaJapan

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