Macrophage surface glycoproteins binding to galectin-3 (Mac-2-antigen)
- 346 Downloads
Galectin-3 (formerly called Mac-2 antigen) is a ∼30 kDa carbohydrate-binding protein expressed on the surface of inflammatory macrophages and several macrophage cell lines. We have purified from lysates of the murine macrophage cell line WEHI-3 glycoproteins that bind to a galectin-3 affinity column. Several of these receptors are labelled after biotinylation of intact cells showing their location at the cell surface. N-terminal aminoacid sequencing of intact galectin-3-binding glycoproteins isolated from preparative SDS-gels or of chemically derived fragments showed several homologies with known proteins and identification was confirmed by immunoprecipitation with specific antibodies. The glycoproteins were shown to be: the α-subunit(CD11b) of the CD11b/CD18 integrin(Mac-1 antigen); the lysosomal membrane glycoproteins LAMPs 1 and 2 which are known in part to be expressed at cell surfaces; the Mac-3 antigen, a mouse macrophage differentiation antigen defined by the M3/84 monoclonal antibody and related immunochemically to LAMP-2; the heavy chain of CD98, a 125 kDa heterodimeric glycoprotein identified by the 4F2/RL388 monoclonal antibodies respectively on human and mouse monocytes/macrophages and on activated T cells. Further studies showed that CD11b/CD18, CD98 and Mac-3 are major surface receptors for galectin-3 on murine peritoneal macrophages elicited by thioglycollate. Abbreviations: PBS, phosphate buffered saline; CNBR, cyanogen bromide; PMSF, phenyl methyl sulphonyl fluoride
Unable to display preview. Download preview PDF.
- 2.Hughes RC (1994) Glycobiol. 4: 5-12.Google Scholar
- 21.Chicheportiche Y, Vassalli P (1994) J Biol Chem 269: 5512-17.Google Scholar
- 22.Vlassara H, Li YM, Imani F, Wojciechowicz D, Yang Z, Liu F-T, Cerami A (1995) Nature Molec Med 1: 634-46.Google Scholar
- 34.Luscher B, Pousseaux M, Lees R, MacDonald HR, Bron C (1986) J Immunol 135, 3951-7.Google Scholar