Advertisement

Biotechnology Letters

, Volume 19, Issue 11, pp 1087–1091 | Cite as

Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph

  • S.H. Ha
  • T.H. Park
Article

Abstract

Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant β-galactosidase in Spodoptera frugipera/ Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph, while host cells began to die 3 days after infection in the medium without hemolymph.

Keywords

Organic Chemistry Recombinant Protein Host Cell Constant Level Efficient Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ha, SH, Park, TH and Kim, S-E (1996). Biotechnol. Tech. 10:401-406Google Scholar
  2. Hara, T, Tarui, H, Kawaguchi, H, Yoshimura, H and Kawarabata, T (1995). J. Seric. Sci. Jpn. 64:477-481Google Scholar
  3. Lee, SH and Park, TH (1994). Biotechnol. Lett. 16:327-332Google Scholar
  4. Lee, SH, and Park, TH. (1995). Biotechnol. Tech. 9:719-724Google Scholar
  5. Licari, P, Jarvis, DL and Bailey, JE (1993). Biotechnol. Prog. 9:146-152Google Scholar
  6. Lindsay, DA and Betenbaugh, MJ (1992). Biotechnol. Bioeng. 39:614-618Google Scholar
  7. Maiorella, B, Inlow, D, Shauger, A and Harano, D (1988). Bio/technol. 6:1406-1410Google Scholar
  8. Miller, JH (1972). Purification of β-galactosidase. In: Experiments in Molecular Genetics, pp 398-404, New York: Cold Spring Harbor Laboratory PressGoogle Scholar
  9. Miltenburger, HG (1982). Investigation on the cultivation of insect cell lines in serum-free media. In: Hormonally Defined Media: A Tool in Cell Biology, G Fisher and RJ Wieser, eds pp 31-43, New York: Springer-VerlagGoogle Scholar
  10. Murhammer, DW and Goochee, CF (1988). Bio/technol. 6:1411-1418Google Scholar
  11. Reuveny, S, Kim, YJ, Kemp, CW and Shiloach, J (1993). Biotechnol. Bioeng. 42:235-239Google Scholar
  12. Wang, M-Y, Kwong, S and Bentley, WE (1993). Biotechnol. Prog. 9:355-361Google Scholar
  13. Wickham, TJ, Davis, T, Granados, RR, Shuler, ML and Wood, HA (1992). Biotechnol. Prog. 8:391-396Google Scholar
  14. Wilkie, GEI, Stockdale, H and Pirt, SV (1980). Develop. Biol. Stand. 46:29-37Google Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • S.H. Ha
    • 1
  • T.H. Park
    • 2
  1. 1.Department of Genetic EngineeringSung Kyun Kwan UniversitySuwonKorea
  2. 2.Department of Chemical EngineeringSeoul National UniversitySeoulKorea

Personalised recommendations