Biotechnology Letters

, Volume 19, Issue 11, pp 1087–1091 | Cite as

Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph

  • S.H. Ha
  • T.H. Park


Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant β-galactosidase in Spodoptera frugipera/ Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph, while host cells began to die 3 days after infection in the medium without hemolymph.


Organic Chemistry Recombinant Protein Host Cell Constant Level Efficient Production 
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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • S.H. Ha
    • 1
  • T.H. Park
    • 2
  1. 1.Department of Genetic EngineeringSung Kyun Kwan UniversitySuwonKorea
  2. 2.Department of Chemical EngineeringSeoul National UniversitySeoulKorea

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