Biotechnology Techniques

, Volume 11, Issue 6, pp 421–422 | Cite as

Separation of inulinase from Kluyveromyces marxianus using reversed micellar extraction

  • Adalberto Pessoa Jr
  • Michele Vitolo


Inulinase from K. marxianus was extracted into a reversed micelle phase of the cationic surfactant BDBAC (n-benzyl-n-dodecyl-n-bis(2-hydroxyethyl)ammonium chloride) in isooctane/hexanol. The extractions carried out with cells (5.9 g/l) presented a recovery yield of 87% and a purification factor 2.8. Similar values were found for inulinase recovered from the clarified medium (91% recovery yield and 2.8 purification factor). For scaled-up (400-fold) extractions, the recovery of the initial activity reached 77% and the enrichment factor was 2.8.


Chloride Ammonium Surfactant Purification Enrichment Factor 
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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Adalberto Pessoa Jr
    • 1
  • Michele Vitolo
    • 2
  1. 1.DEBIQ/FAENQUILLorena/SPBrazil
  2. 2.Biochemical and Pharmaceutical Dept./FCF/USPSao Paulo/SPBrazil

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