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Biotechnology Techniques

, Volume 11, Issue 6, pp 421–422 | Cite as

Separation of inulinase from Kluyveromyces marxianus using reversed micellar extraction

  • Adalberto Pessoa Jr
  • Michele Vitolo
Article

Abstract

Inulinase from K. marxianus was extracted into a reversed micelle phase of the cationic surfactant BDBAC (n-benzyl-n-dodecyl-n-bis(2-hydroxyethyl)ammonium chloride) in isooctane/hexanol. The extractions carried out with cells (5.9 g/l) presented a recovery yield of 87% and a purification factor 2.8. Similar values were found for inulinase recovered from the clarified medium (91% recovery yield and 2.8 purification factor). For scaled-up (400-fold) extractions, the recovery of the initial activity reached 77% and the enrichment factor was 2.8.

Keywords

Chloride Ammonium Surfactant Purification Enrichment Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Adalberto Pessoa Jr
    • 1
  • Michele Vitolo
    • 2
  1. 1.DEBIQ/FAENQUILLorena/SPBrazil
  2. 2.Biochemical and Pharmaceutical Dept./FCF/USPSao Paulo/SPBrazil

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