Experimental & Applied Acarology

, Volume 21, Issue 5, pp 279–291 | Cite as

Detection of Theileria annulata by the PCR in ticks (Acari: Ixodidae) collected from cattle in Mauritania

  • C. d'Oliveira
  • M. van der Weide
  • P. Jacquiet
  • F. Jongejan
Article

Abstract

We report on the detection of Theileria annulata in infected Hyalomma ticks by the PCR using primers derived from the gene encoding the 30 kDa major merozoite surface antigen (Tams1–1). No inhibition of the PCR was observed and as little as 0.1 pg of parasite DNA, corresponding to 12 sporozoites, could be detected in non-infected tick DNA samples, spiked with T. annulata genomic DNA. Hyalomma dromedarii ticks, fed on a calf experimentally infected with T. annulata, were used to validate the PCR further. The infection rate in the adult ticks, fed as nymphs during the febrile reaction, was high (62%), dropped to zero for 1 day in tick batches that engorged after treatment with ButalexTM and increased to 30% 2 days later and 38% of the ticks acquired the infection after feeding as nymphs during a carrier state piroplasm parasitaemia of less than 0.1%. As an internal control, 16S tick rDNA sequences could be amplified from T. annulata-negative tick samples. Finally, 202 adult ticks from Mauritania, collected from zebu cattle carrying low levels of Theileria piroplasms, were tested by the PCR. Thirty-eight out of 52 (73%) and 17 out of 30 (57%) H. dromedarii from the Gorgol and Trarza regions, respectively and two out of 30 (7%) Hyalomma marginatum rufipes from the Gorgol region were positive. Hyalomma marginatum rufipes, Rhipicephalus evertsi evertsi and Rhipicephalus guilhoni from the Trarza region were negative. These findings confirm that H. dromedarii is the main vector of T. annulata in Mauritania and that the PCR is a useful method of determining the infection rates in ticks collected from cattle carrying low levels of T. annulata piroplasms.

Hyalomma ticks PCR Tams1–1 Theileria annulata Mauritania 

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REFERENCES

  1. Allsopp, B.A., Baylis, H.A., Allsopp, M.T.E.P., Cavalier-Smith, T., Bishop, R.P., Carrington, D.M., Sohanpal, B. and Spooner, P. 1993. Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitology, 107: 157–165.Google Scholar
  2. Black IV, W.C. and Piesman, J. 1994. Phylogeny of hard-and soft-tick taxa (Acari:Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl. Acad. Sci., USA 91: 10034–10038.Google Scholar
  3. Clary, D.O. and Wolstenholme, D.R. 1985. The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J. Mol. Evol. 22: 252–271.Google Scholar
  4. Conrad, P.A., Iams, K., Brown, W.C., Sohanpal, B. and ole-MoiYoi, O.K. 1987. DNA probes detect genomic diversity in Theileria parva stocks. Mol. Biochem. Parasitol. 25: 213–226.Google Scholar
  5. Daubney, R. and Said, M.S. 1951. Egyptian fever of cattle, transmission of Theileria annulata by Hyalomma excavatum. Parasitology, 41: 249–260.Google Scholar
  6. De Kok, J.B., d'Oliveira, C. and Jongejan, F. 1993. Detection of the protozoan parasite Theileria annulata in Hyalomma ticks by the polymerase chain reaction. Exp. Appl. Acarol. 17: 839–846.Google Scholar
  7. D'Oliveira, C., van der Weide, M., Habela, M.A., Jacquiet, P. and Jongejan, F. 1995. Detection of Theileria annulata in blood samples of carrier cattle by PCR. J. Clin. Microbiol. 33: 2665–2669.Google Scholar
  8. Dschunkowsky, E. and Urodschevich, V. 1924. Theileriasis in goats, sheep and cattle with a description of Theileria hirci (nov. sp.) from Serbia. Parasitology 16: 107–110.Google Scholar
  9. Gage, K.L., Gilmour, R.D., Karstens, R.H. and Schwan, T.G.S.O. 1992. Detection of Rickettsia rickettsii in saliva, haemolymph and triturated tissues of infected Dermacentor andersoni ticks by polymerase chain reaction. Mol. Cell. Probes 6: 333–341.Google Scholar
  10. Higgins, J.A. and Azad, A.F. 1995. Use of polymerase chain reaction to detect bacteria in arthropods: a review. J. Med. Entomol. 32: 213–222.Google Scholar
  11. Jacquiet, P., Dia, M.L., Perié, N.M., Jongejan, F., Uilenberg, G. and Morel, P.C. 1990. Presénce de Theileria annulata en Mauretanie. Rev. Elev. Méd. Vét. Pays Trop. 43: 489–490.Google Scholar
  12. Jacquiet, P., Colas, F., Cheikh, D., Thiam, E. and Ly, B.A. 1994. Epidémiologie descriptive de la theilériose bovine a Theileria annulata en Mauritanie, Afrique de l'Ouest sub-saharienne. Rev. Elev. Méd. Vét. Pays Trop. 47: 147–155.Google Scholar
  13. Johnson, B.J.B., Happ, C.M., Mayer, L.W. and Piesman, J. 1992. Detection of Borrelia burgdorferi in ticks by species-specific amplification of the flagellin gene. Am. J. Trop. Med. Hyg. 47: 730–741.Google Scholar
  14. Jongejan, F., Morzaria, S.P., Um el Hassan Mustafa and Latif, A.A. 1983. Infection rates of Theileria annulata in the salivary glands of the tick Hyalomma marginatum rufipes. Vet. Parasitol. 13: 121–126.Google Scholar
  15. Kawazu, S., Kamio, T., Sekizaki, T. and Fujisaki, K. 1995. Theileria sergenti and Theileria buffeli: PCR-based marker system for differentiating the parasite species from the infected cattle blood and infected tick salivary gland. Exp. Parasitol. 81: 430–435.Google Scholar
  16. McHardy, N., Wekesa, L.S., Hudson, A.T. and Randall, A.S. 1985. Antitheilerial activity of BW720C (buparvaquone): a comparison with parvaquone. Res. Vet. Sci. 39: 29–33.Google Scholar
  17. Mazlum, Z. 1969. Transmission of Theileria annulata by the crushed infected unfed Hyalomma dromedarii. Parasitology 59: 597–600.Google Scholar
  18. Morel, P.C. and Uilenberg, G. 1981. The nomenclature of some Theileria spp. (Sporozoa, Babesioidea) of domestic ruminants. Rev. Elev. Méd. Vét. Pays Trop. 34: 139–143.Google Scholar
  19. Persing, D.H., Telford III, S.R., Spielman, A. and Barthold, S.W. 1990. Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the polymerase chain reaction. J. Clin. Microbiol. 28: 566–572.Google Scholar
  20. Peter, T.F., Deem, S.L., Barbet, A.F., Norval, R.A.I., Simbi, B.H., Kelly, P.J. and Mahan, S.M. 1995. Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe. J. Clin. Microbiol. 33: 166–172.Google Scholar
  21. Robinson, P.M. 1982. Theileria annulata and its transmission — a review. Trop. Animal Health Prod. 14: 3–12.Google Scholar
  22. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  23. Schein, E., Buscher, G. and Friedhoff, K.T. 1975. Lichtmikroskopie Untersuchungen überdie Entwicklung von Theileria annulata in Hyalomma anatolicum. Z. Parasitenkunde, 48: 123–126.Google Scholar
  24. Schein, E., Rehbein, G., Voigt, W.P. and Zweygarth, E. 1981. Babesia equi (Laveran, 1901). 1. Development in horses and in lymphocyte culture. Z. Tropenmed. Parasitol. 32: 227–233.Google Scholar
  25. Sergent, E., Donatien, A., Parrot, L. and Lestoguard, F. 1928. Tiques et piroplasmoses bovines d'Algerie. Bull. Soc. Pathol. Exot. 21: 846–849.Google Scholar
  26. Shiels, B.R., Smyth, A., Dickson, J., McKellar, S., Tetley, L., Fujisaki, K., Hutchinson, B. and Kinnaird, J.H. 1994. A stoichiometric model of stage differentiation in the protozoan parasite Theileria annulata. Mol. Cell. Diff. 2: 101–125.Google Scholar
  27. Shiels, B.R., d'Oliveira, C., McKellar, S., Ben-Miled, L., Kawazu, S. and Hide, G. 1995. Selection of diversity at putative glycosylation sites in the immunodominant merozoite/piroplasm surface antigen of Theileria parasites. Mol. Biochem. Parasitol. 72: 149–162.Google Scholar
  28. Stich, R.W., Bantle, J.A., Kocan, K.M. and Fekete, A. 1993. Detection of Anaplasma marginale (Rickettsiales, Anaplasmataceae) in haemolymph of Dermacentor andersoni (Acari, Ixodidae) with the polymerase chain reaction. J. Med. Entomol. 30: 781–788.Google Scholar
  29. Tait, A. and Hall, F.R. 1990. Theileria annulata: control measures, diagnosis and the potential use of subunit vaccines. Rev. Sci. Tech. Off. Int. Epiz. 9: 387–403.Google Scholar
  30. Uilenberg, G. 1981. Theilerial species of domestic livestock. In Advances in the control of theileriosis, A.D. Irvin, M.P. Cunningham, and A.S. Young (eds), pp 4–37. Martinus Nijhoff, The Hague, The Netherlands.Google Scholar
  31. Um el Hassan Mustafa, Jongejan, F. and Morzaria, S.P. 1983. Note on the transmission of Theileria annulata by Hyalomma ticks in the Sudan. Vet. Quart. 5: 112–113.Google Scholar
  32. van Straten, M. and Jongejan, F. 1993. Ticks (Acari: Ixodidae) infesting the Arabian camel (Camelus dromedarius) in the Sinai, Egypt with a note on the acaricidal efficacy of Ivermectin. Exp. Appl. Acarol. 17: 605–616.Google Scholar
  33. Walker, A.R., Fletcher, J.D., McKellar, S.B., Bell, L.J. and Brown, C.G.D. 1985. The maintainance and survival of Theileria annulata in colonies of Hyalomma anatolicum anatolicum. Ann. Trop. Med. Parasitol. 79: 199–209.Google Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • C. d'Oliveira
    • 1
  • M. van der Weide
    • 1
  • P. Jacquiet
    • 2
  • F. Jongejan
    • 1
  1. 1.Department of Parasitology and Tropical Veterinary MedicineUtrecht UniversityUtrechtThe Netherlands
  2. 2.Service de ParasitologieCentre d'Elevage et de Recherches Ve´te´rinairesNouakchottMauritania

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