Chromosome Research

, Volume 5, Issue 7, pp 451–456

Karyotype analysis of Helianthus annuus using Giemsa banding and fluorescence in situ hybridization

  • Otto Schrader
  • Richard Ahne
  • Jorg Fuchs
  • Ingo Schubert
Article

DOI: 10.1023/A:1018412912790

Cite this article as:
Schrader, O., Ahne, R., Fuchs, J. et al. Chromosome Res (1997) 5: 451. doi:10.1023/A:1018412912790

Abstract

The karyotype of H. annuus was analysed by computer-aided image processing with respect to the chromosome length, arm ratio, occurrence and chromosomal position of intercalary heterochromatin and the location of 18S/25S and 5S ribosomal RNA genes. The karyotype was subdivided into a group of four acrocentric chromosome pairs, of which two were distinguishable by HKG (HCl, KOH, Giemsa) banding and a group of 13 meta- to submetacentric pairs. The latter could be subdivided into seven pairs with one and six pairs with two HKG bands. Three pairs of submetacentric satellite chromosomes revealed 18S/25S rDNA loci after fluorescence in situ hybridization (FISH) and silver staining . A fourth, smaller and possibly inactive, locus occurred in the terminal position on a metacentric pair. One submetacentric satellite chromosome pair revealed a 5S rRNA gene locus in the pericentromeric position; a second locus marked a submetacentric pair with one HKG band. The C-banding technique marks exclusively centromeric heterochromatin. Measurements of chromosomes in combination with Giemsa banding and FISH enabled the discrimination of most chromosome pairs of the sunflower.

computer-aided karyotyping FISH Giemsa banding Helianthus nucleolar organizing region rDNA 5S rRNA genes silver staining 

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Otto Schrader
    • 1
  • Richard Ahne
    • 1
  • Jorg Fuchs
    • 2
  • Ingo Schubert
    • 2
  1. 1.Institut fur Zuchtungsmethodik bei Gemuse der Bundesanstalt fur Zuchtungsforschung an KulturpflanzenQuedlinburgGermany
  2. 2.Institut fur Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany

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