Biotechnology Techniques

, Volume 11, Issue 9, pp 671–674

Rapid identification of biosurfactant-producing bacterial strains using a cell surface hydrophobicity technique

  • V. Pruthi
  • S.S. Cameotra

DOI: 10.1023/A:1018411427192

Cite this article as:
Pruthi, V. & Cameotra, S. Biotechnology Techniques (1997) 11: 671. doi:10.1023/A:1018411427192


Rapid identification of biosurfactant-producing bacterial strains was achieved by assaying cell surface hydrophobicity which had a direct correlation with biosurfactant production by Serratia marcescens, Pseudomonas aeruginosa, Bacillus pumilus, B. laterosporus, Acineto- bacter calcoaceticus, Escherichia coli and Staphylococcus aureus. These properties namely, Hydrophobic Interaction Chromatography, Salt Aggregation Test, Bacterial Adherence To Hydrocarbon and adhesion to polystyrene by Replica Plate test, provide a simple means for identifying bacteria associated with the production of biosurfactants.

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • V. Pruthi
    • 1
  • S.S. Cameotra
    • 1
  1. 1.Institute of Microbial Technology, Sec. 39AChandigarhIndia

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