Biotechnology Techniques

, Volume 11, Issue 7, pp 497–501

The displacement effect of phenylalanine on lysozyme elution in hydrophobic interaction chromatography

  • R.J. Klimchak
  • S. Wang
Article

Abstract

The volume, retention time, and shape of the lysozyme peak eluted from a hydrophobic interaction chromatography column (TosoHaas 650 M Phenyl) was influenced by the presence and concentration of phenylalanine in the elution buffer. Lysozyme peak retention time decreased by a factor of 2.5 with the addition of 86 mM phenylalanine to the elution buffer.

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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • R.J. Klimchak
    • 1
  • S. Wang
    • 1
  1. 1.Department of Chemical and Biochemical EngineeringReuters UniversityNew BrunswickUSA

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