Biotechnology Letters

, Volume 19, Issue 11, pp 1125–1127

Toxicity of hardwood extractives toward Saccharomyces cerevisiae glucose fermentation

  • T.D. Ranatunga
  • J. Jervis
  • R.F. Helm
  • J.D. McMillan
  • C. Hatzis
Article

Abstract

The toxicity of oak and yellow-poplar wood extracts, as well as a first-stage hardwood hydrolyzate liquor prepared from a red oak:white oak:yellow-poplar (1:1:1) sawdust, on Saccharomyces cerevisiae D5A was examined. Acetone/water and hot methanol extracts of solid biomass samples from white oak, red oak, and yellow-poplar gave 88-94% of the ethanol produced with controls. The organism was tolerant to the compounds present in the xylose-rich hydrolyzate, with fermentation efficiency being improved to 97% of that obtained with controls by using an overliming/thermal conditioning protocol.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Antal, MJ, Leesomboon, T, Mok, WS and Richards, GN (1991). Carbohydr. Res., 217: 71-85.Google Scholar
  2. Delgenes, JP, Moletta, R and Navarro, JM (1996). Enzyme Microb. Technol., 19: 220-225.Google Scholar
  3. Frazer, FR and McCaskey, TA (1989). Biomass, 18: 31-42.Google Scholar
  4. Hahn-Hägerdal, B (1996). Appl. Biochem. Biotechnol. 57/58: 195-199.Google Scholar
  5. Ingram, LO, Lai, X, Moniruzzaman, M, Wood, BE and York, SW (1997). Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria. In: Fuels and Chemicals from Biomass, BC Saha and J Woodward, eds ACS Symp. Ser., 666: 57-73, American Chemical Society, Washington D. C.Google Scholar
  6. McMillan, JD (1994). Conversion of Hemicellulose Hydrolyzates to Ethanol. In: Enzymatic Conversion of Biomass for Fuels Production, ME Himmel, JO Baker and RP Overend, eds ACS Symp. Ser., 566: 411-437, American Chemical Society, Washington D.C.Google Scholar
  7. Ranatunga, TD, Jervis, J, Helm, RF, McMillan, JD, Hatzis, C (1997). Appl. Biochem. Biotechnol., in press.Google Scholar
  8. Tran, AV and Chambers, RP (1986). Enzyme Microb. Technol., 8: 439-444.Google Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • T.D. Ranatunga
    • 1
  • J. Jervis
    • 1
  • R.F. Helm
    • 1
  • J.D. McMillan
    • 2
  • C. Hatzis
    • 2
  1. 1.Fralin Biotechnology Center, Department of Wood Science and Forest ProductsVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  2. 2.Biotechnology Center for Fuels and ChemicalsNational Renewable Energy LaboratoryGoldenUSA

Personalised recommendations