Biotechnology Letters

, Volume 19, Issue 5, pp 433–435

Inhibition of alkaline phosphatase by surfactin, a natural chelating lipopeptide from Bacillus subtilis

  • Muriel Bortolato
  • Françoise Besson
  • Bernard Roux
Article

Abstract

Surfactin, an acidic lipopeptide of Bacillus subtilis, at 70 μM non-competitively inhibited alkaline phosphatase without its glycosyl-phosphatidylinositol anchor by 50%. Enzyme activity was restored by adding 50 μM ZnCl 2 and 1 mM MgCl2 confirming the chelating action of the free carboxyl groups of the Asp and Glu residues in the peptide moiety of surfactin.

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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Muriel Bortolato
    • 1
  • Françoise Besson
    • 1
  • Bernard Roux
    • 1
  1. 1.Laboratoire de Physico-Chimie Biologique, UPRESA-CNRS 5013Université Claude Bernard Lyon IVilleurbanne CedexFrance

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