Biotechnology Letters

, Volume 19, Issue 5, pp 433–435

Inhibition of alkaline phosphatase by surfactin, a natural chelating lipopeptide from Bacillus subtilis

  • Muriel Bortolato
  • Françoise Besson
  • Bernard Roux


Surfactin, an acidic lipopeptide of Bacillus subtilis, at 70 μM non-competitively inhibited alkaline phosphatase without its glycosyl-phosphatidylinositol anchor by 50%. Enzyme activity was restored by adding 50 μM ZnCl 2 and 1 mM MgCl2 confirming the chelating action of the free carboxyl groups of the Asp and Glu residues in the peptide moiety of surfactin.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Angrand M. (1997) Thesis, no 030-97, UCB, Lyon I, France.Google Scholar
  2. Arima K., Kakinuma A. and Tamura G. (1968) Biophys. Res. Commun. 31, 488–494.CrossRefGoogle Scholar
  3. Bernheimer A.W. and Avigad L.S. (1970) J. Gen. Microbiol. 61, 361–369.PubMedGoogle Scholar
  4. Besson F., Hourdou M.L. and Michel G. (1990) Biochim. Biophys. Acta 1036, 101–106.PubMedGoogle Scholar
  5. Besson F., Tenoux I., Hourdou M.L. and Michel G. (1992) Biochim. Biophys. Acta 1123, 51–58.PubMedGoogle Scholar
  6. Coleman J.E. (1992) Annu. Rev. Biophys. Biomol. Struct. 21, 441–483.PubMedCrossRefGoogle Scholar
  7. Cyboron G.W. and Wuthier R.E. (1981) J. Biol. Chem. 256, 7262–7268.PubMedGoogle Scholar
  8. Dixon M. and Webb C. (1964) Enzymes, 2nd edn, Longmans Green, London 327–331.Google Scholar
  9. Holmquist B. (1988) Methods in Enzymol. 158, 6–12.CrossRefGoogle Scholar
  10. Hosono K. and Suzuki H. (1983a) J. Antibiotics 36, 667–673.Google Scholar
  11. Hosono K. and Suzuki H. (1983b) J. Antibiotics 36, 673–678.Google Scholar
  12. Hosono K. and Suzuki H. (1983c) J. Antibiotics 36, 679–683.Google Scholar
  13. Itokawa H., Miyashita T., Morita H., Takeya K., Hirano T., Homma M. and Oka K. (1994) Chem. Pharm. Bull. 42, 604–607.PubMedGoogle Scholar
  14. Kameda Y., Ouhira S., Matsui K., Kanatomoto S., Hase T. and Atsusaka T. (1974) Chem. Pharm. Bull. 22, 938–944.PubMedGoogle Scholar
  15. Kim E.E. and Wyckoff H.W. (1991) J. Mol. Biol. 218, 449–464.PubMedCrossRefGoogle Scholar
  16. Low M.G. (1989) Biochem. Biophys. Acta, 988, 427–454.PubMedGoogle Scholar
  17. Radisson J., Angrand M., Chavassieux P., Roux B. and Azzar G. (1996) Int. J. Biochem. Cell. Biol. 28, 421–430.PubMedCrossRefGoogle Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Muriel Bortolato
    • 1
  • Françoise Besson
    • 1
  • Bernard Roux
    • 1
  1. 1.Laboratoire de Physico-Chimie Biologique, UPRESA-CNRS 5013Université Claude Bernard Lyon IVilleurbanne CedexFrance

Personalised recommendations