Intergeneric hybridization between Brassica species and Crambe abyssinica
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In crosses of B. napus × C. abyssinica and B. campestris × C. abyssinica, pre-fertilization incompatibility was observed. In crosses of B. juncea × C. abyssinica, pollen grains of C. abyssinica were compatible to the stigmas of all the B. juncea pistils, the pollen tube growth was normal and the pollen tube reached the micropyle in 3–4 days after pollination. Through comparative studies on embryo development in intergeneric crosses of B. juncea × C. abyssinica and self-pollination of B. juncea, the causes of the hybrid embryo abortion were found. An intergeneric hybrid between B. juncea × C. abyssinica was obtained for the first time through conventional crossing method combined with ovary culture, when C. abyssinica was used as a paternal parent. The hybridity was confirmed by morphology, cytology and isozyme analysis.
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