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Biotechnology Letters

, Volume 19, Issue 8, pp 755–758 | Cite as

Skin unhairing proteases of Bacillus subtilis: production andpartial characterization

  • Hermosinda Varela
  • Mario Daniel Ferrari
  • Leopoldo Belobrajdic
  • Adriana Vazquez
  • Milka Lyliam Loperena
Article

Abstract

Proteases able to unhair sheep skins were produced from Bacillus subtilis. Protease activity was increased from 640 to 990 U/mL by using a fed-batch culture with glucose added sequentially up to 10 g/L. The crude enzyme prep-aration was mainly a mixture of metallo- and serine-proteases with optimal pH and temperature for protease activity at 7 and 50-55°C, respectively. At pH 8 and 30°C, protease activity of the crude enzyme was 33% of the maximal value and 97% of the original activity (900 U/mL) was retained after incubation for 4 hours.

Keywords

Glucose Enzyme Organic Chemistry Bacillus Bacillus Subtilis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Hermosinda Varela
    • 1
  • Mario Daniel Ferrari
    • 1
  • Leopoldo Belobrajdic
    • 1
  • Adriana Vazquez
    • 1
  • Milka Lyliam Loperena
    • 1
  1. 1.Depto. Bioingeniería, Instituto de Ingeniería Química, Facultad de IngenieríaUniversidad de la RepúblicaMontevideoUruguay

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