, Volume 103, Issue 1, pp 75–82 | Cite as

Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers

  • My. Hassan Sedra
  • Philippe Lashermes
  • Pierre Trouslot
  • Marie-Christine Combes


Genetic variation among 43 date palm (Phoenix dactylifera L.) accessions, including 37 accessions from Morocco and 6 cultivars from Iraq and Tunisia, was studied using Random Amplified Polymorphic DNA (RAPD) markers. The pre-screening of 123 primers on four genotypes allowed selection of 19 primers which revealed polymorphism and gave reproducible results. All 43 analysed genotypes were distinguishable by their band patterns. RAPD technology therefore appears very effective for identifying accessions of date palm. RAPD-based genetic distance was used to determine the relationships between the accessions. The grouping-association identified by cluster analysis was rather weak. However, morphologically similar varieties clustered together. A relatively low polymorphism and a lack of evident organisation are observed among the date palm varieties grown in Morocco. This could be related to the mode of introduction and maintenance of the Moroccan date palm germplasm involving limited foundation germplasm, exchange of cultivars between plantations, and periodic development of new recombinant cultivars following sexual reproduction.

cultivars date palm genetic diversity Morocco RAPD markers 


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  1. Aît Chitt, M., S.H. Mantell, M. Thangavelu & C.C. Ainsworth, 1995. Cloning date palm (Phoenix dactyliferaL.) DNA and characterization of low, medium and high copy DNA sequences. Elaeis (7)1: 57-63.Google Scholar
  2. Baaziz, M. & M. Saaidi, 1988. Preliminary identification of date palm cultivars by esterase isoenzymes and peroxidase activities. Can J Bot 66: 89-93.Google Scholar
  3. Bendiab, K., M. Baaziz, Z. Brakez & My.H. Sedra, 1993. Correlation of isoenzyme polymorphism and Bayoud-disease resistance in date palm cultivars and progeny. Euphytica 65: 23-32.CrossRefGoogle Scholar
  4. Bennaceur, M., C. Lanaud, M.H. Chevalier & N. Bounagua, 1991. Genetic diversity of the date palm (Phoenix dactyliferaL.) from Algeria revealed by enzyme markers. Plant Breed 107: 56-69.CrossRefGoogle Scholar
  5. Benzecri, J.P., 1973. L'analyse des données. Tome 2, L'analyse des correspondances. Dunod, Paris.Google Scholar
  6. Brac de la Perrière, R.A. & A. Benkhalifa, 1991. Progression de la fusariose du palmier dattier en Algérie. Sécheresse 2: 119-128.Google Scholar
  7. Corniquel, B. & L. Mercier, 1994. Date palm (Phoenix dactyliferaL.) cultivar identification by RFLP and RAPD. Plant Sci 101: 163-172.Google Scholar
  8. Djerbi, M., H. Aouad, H. El Filali, M. Saaidi, A. Chtioui, My.H. Sedra, M. Allaoui, T. Hamdaoui & M. Oubrich, 1986. Preliminary results on selection of high quality Bayoud resistant clones among natural date palm population in Morocco. In: Second Symposium on Date Palm, 3-6 March 1986, King Faisal University, Al-Hassa, Saudi Arabia, pp. 386-399.Google Scholar
  9. Fakir, S., J. Carbonnier & A. Birouk, 1992. Analyse du polymorphisme enzymatique et protéique des cultivars marocains du palmier dattier (Phoenix dactyliferaL.). In: Complexes d'espèces, flux de gènes et ressources génétiques des plantes. Actes du Colloque International, 8-10/1/92, Paris Lavoisier, pp. 645.Google Scholar
  10. Hu, J. & C.F. Quiros, 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10: 505-511.CrossRefGoogle Scholar
  11. Koller, B., A. Lehmann, J.M. McDermott & C. Gessler, 1993. Identification of apple cultivars using RAPD markers. Theor Appl Genet 85: 901-904.CrossRefGoogle Scholar
  12. Louvet J. & G. Toutain, 1973. Recherches sur les fusarioses. VII. Nouvelles observations sur la fusariose du palmier dattier et précisions concernant la lutte. Ann Phytopathol 5: 35-52.Google Scholar
  13. Lashermes, P., J. Cros, P. Marmey & A. Charrier, 1993. Use of random amplified polymorphic DNA markers to analyse genetic variability and relationships of Coffeaspecies. Crop Evol Genet Res 40: 91-99.CrossRefGoogle Scholar
  14. Mossler, A., K.N. Egger & G.A. Hughes, 1992. Low levels of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers. Can J For Res 22: 1332-1337.CrossRefGoogle Scholar
  15. Pereau-Leroy, P., 1958. Le palmier dattier au Maroc. Ministère de l'Agriculture (142 pp.). Instit Franc Rech Outre-mer, Paris.Google Scholar
  16. Saaidi, M., G. Toutain, H. Bannerot & J. Louvet, 1981. La sélection du palmier dattier (Phoenix dactyliferaL.) pour la résistance au Bayoud. Fruits 36: 241-249.Google Scholar
  17. Sedra, My.H., 1992. Evaluation and selection of the resistant good cultivars and clones of date palm of the Bayoud disease. Arab Soc Plant Prot 2: 155-160.Google Scholar
  18. Sedra, My.H., 1995. Triage d'une collection de génotypes de palmier dattier pour la résistance au Bayoud causé par Fusarium oxysporumf.sp. albedinis. Al Awamia 90: 9-18.Google Scholar
  19. Sedra, My.H., H. El Filali & D. Frira, 1993. Observations sur quelques caractéristiques phénotypiques et agronomiques du fruit des variétés et clones du palmier dattier sélectionnés. Al Awamia 82: 105-120.Google Scholar
  20. Sedra, My.H., H. El Filali, A. Benzine, M. Allaoui, S. Nour & Z. Boussak, 1996. La palmeraie dattière marocaine: Evaluation du patrimoine phénicicole. Fruits 1: 247-259.Google Scholar
  21. Toutain, G., A. Bachra & A. Chari, 1971. Cartographie variétale de la palmeraie marocaine (242 pp.). Direc Rech Agron, Rabat, Morocco.Google Scholar
  22. Van der Peer, Y. & R. De Wachter, 1993. TREECON: a software package for the construction and drawing of evolutionary trees. Comput Applic Biosci 9: 177-182.Google Scholar
  23. Welsh, J. & M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acid Res 18: 7213-7218.Google Scholar
  24. Williams, J.G.K., A.R. Kubelik, J.L. Kenneth, J.A. Rafalski & S.V. Tingy, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 18: 6522- 6531.Google Scholar
  25. Wolff, K. & J.P. Van Run, 1993. Rapid detection of genetic variability in chrysanthemum (Dendranthema grandifloraTzvelev) using random primers. Heredity 71: 335-341.PubMedGoogle Scholar
  26. Wrigley, G., 1995. Date palm. In: J. Smartt & N.W. Simonds (Eds.), Evolution of Crop Plants, 2nd ed., pp. 399-403. Longman, London.Google Scholar
  27. Yang, X. & C. Quiros, 1993. Identification and classification of celery cultivars with RAPD markers. Theor Appl Genet 86: 205-212.CrossRefGoogle Scholar

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© Kluwer Academic Publishers 1998

Authors and Affiliations

  • My. Hassan Sedra
  • Philippe Lashermes
  • Pierre Trouslot
  • Marie-Christine Combes

There are no affiliations available

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