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Studies on somaclonal variants for resistance to scab in bread wheat (Triticum aestivum L.) through in vitro selection for tolerance to deoxynivalenol

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Abstract

This study was conducted to develop an efficient in vitro selection system for scab resistance by using in vitro screening for tolerance to deoxynivalenol (DON). Immature embryos of two wheat varieties, a scab-resistant variety Sumai 3 and a susceptible variety Mianyang 11, and their reciprocal F1 hybrids were cultured on MS medium supplemented with 2,4-D 2 mg/l and 0.6 × 10-4 M DON for callus induction. The responses of callus induction and plant regeneration to 0.6 × 10-4 M DON differed significantly between resistant and susceptible varieties, according to observed scab resistance levels at the plant level in the field. The percentage of callus formation of resistant variety Sumai 3 on induction medium containing DON was higher than that of susceptible variety Mianyang 11. Regeneration of DON-tolerant calli on DON-containing differentiation medium differed significantly between Sumai 3 and Mianyang 11. Averaged across the DON-tolerant calli of two varieties and their reciprocals, regeneration of DON-tolerant calli was decreased 3-fold on DON-containing medium. By an inoculation test with conidiospores of Fusarium graminearum Schw, we obtained several resistant lines from progenies of regenerated plants from DON-tolerant calli. These somaclonal lines had lower disease scoring (reaction index, infected spikelets and disease incidence), shorter plants and better yield components than Sumai 3, a famous Chinese resistant variety.

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Author notes

  1. Zhuping Yang

    Present address: Agriculture and Agri-Food Canada, Research Centre, Lacombe, Alberta, T4L 1W1, Canada

Authors and Affiliations

  1. Shanghai Academy of Agricultural Sciences, Agro-Biotechnology Research Center, Shanghai, 201106, P.R. China

    Zhuping Yang & Xiaoyan Yang

  2. Shanghai Academy of Agricultural Sciences, Institute of Plant Protection, Shanghai, 201106, P.R. China

    Dechong Huang

Authors
  1. Zhuping Yang
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  2. Xiaoyan Yang
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  3. Dechong Huang
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Yang, Z., Yang, X. & Huang, D. Studies on somaclonal variants for resistance to scab in bread wheat (Triticum aestivum L.) through in vitro selection for tolerance to deoxynivalenol. Euphytica 101, 213–219 (1998). https://doi.org/10.1023/A:1018354606939

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  • DOI: https://doi.org/10.1023/A:1018354606939

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