, Volume 103, Issue 2, pp 227–234 | Cite as

Chromosomal regions controlling anther culturability in rice (Oryza sativa L.)

  • Masumi Yamagishi
  • Motoyasu Otani
  • Mariko Higashi
  • Yoshimichi Fukuta
  • Kiichi Fukui
  • Takiko Shimada


Diallel analysis has revealed that anther culturability in rice (Oryza sativa L.) is a quantitative trait controlled by the nuclear genome. Mapping of anther culturability is important to increase the efficiency for green plant regeneration from microspores. In the previous study, we detected distorted segregation of RFLP markers in rice populations derived from the anther culture of an F1 hybrid between a japonica cultivar ‘Nipponbare’ and an indica cultivar ‘Milyang 23’. To clarify the association between chromosomal regions showing distorted segregation and anther culturability, the anther culturability of doubled haploid lines derived from the same cross combination was examined, and the association between alleles of the RFLP markers, which exhibiting the most distorted segregation on chromosomes 1, 3, 7, 10 and 11, and the anther culturability was evaluated. One region on chromosome 1 was found to control callus formation from microspores, and one region on chromosome 10 appeared to control the ratio of green to albino regenerated plants. In both regions, the Nipponbare allele had positive effects. Three regions on chromosomes 3, 7 and 11, however, showed no significant effect on anther culturability.

albino plant regeneration anther culture callus formation distorted segregation Oryza sativa L. RFLP mapping 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Masumi Yamagishi
    • 1
  • Motoyasu Otani
    • 1
  • Mariko Higashi
    • 1
    • 2
  • Yoshimichi Fukuta
    • 3
  • Kiichi Fukui
    • 3
  • Takiko Shimada
    • 4
  1. 1.Nonoichi-machiResearch Institute of Agricultural Resources, Ishikawa Agricultural CollegeIshikawaJapan
  2. 2.Kaga Agricultural Extension Center, Saiwai, KagaIshikawaJapan
  3. 3.Hokuriku National Agricultural Experiment Station, MAFF, Inada, JoetsuNiigataJapan
  4. 4.Rice Genome Research ProgramNational Institute of Agrobiological Resources, MAFF, Kannondai, TsukubaIbarakiJapan

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