Biotechnology Letters

, Volume 19, Issue 5, pp 487–490

Enhancement of apparent resistance to ethanol in Lactobacillus hilgardii

  • José António Couto
  • Cristina Pina
  • Tim Hogg
Article

Abstract

The survival of Lactobacillus hilgardii, a highly ethanol-tolerant organism, after an ethanol challenge at 25% (v/v) for 10 min, increased by several log cycles when cells, grown in the absence of ethanol, were pre-treated with 10% (v/v) ethanol, 15% (v/v) methanol or 2% (v/v) butanol for 4 h. A temperature upshift (25 to 40°C) before ethanol challenge demonstrated a similar enhancement of apparent resistance to ethanol. Ethanol shock enhanced apparent resistance to methanol, butanol and heat challenges. The addition of chloramphenicol to cells prior to any pre-treatment did not significantly diminish the increase in ethanol tolerance, suggesting that de novo protein synthesis is not required for induced tolerance in this organism.

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References

  1. Adams M.R. and Moss M.O. (1995). The microbiology of food preservation. In Food Microbiology, The Royal Society of Chemistry, Cambridge UK, pp 55–100.Google Scholar
  2. Arnosti S.N., Singer V.L. and Chamberlin M. (1986). J. Bacteriol. 168, 1243–1249.PubMedGoogle Scholar
  3. Borkovich C.A., Farrely F.W., Finkelstein D.B., Taulien J. and Lindquist S. (1989). Mol. Cel. Biol. 9, 3919–3930.Google Scholar
  4. Boutibonnes P., Tranchard C., Hartke A., Thammavongs B. and Auffray Y. (1992). Int. J. Food Microbiol. 16, 227–236.PubMedCrossRefGoogle Scholar
  5. Boutibonnes P., Giard J.C., Hartke A., Thamavongs B. and Auffray Y. (1993) Antonie van Leeuwenhoek 64, 47–55.PubMedCrossRefGoogle Scholar
  6. Couto J.A. and Hogg T. (1994). J. Appl. Bacteriol. 76, 487–491.Google Scholar
  7. Gold R.S., Meagher M.M., Hutkins R. and Conway T. (1992). J. Ind. Microbiol. 10, 45–54.CrossRefGoogle Scholar
  8. Groat R.G. and Matin A. (1986). J. Ind. Microbiol. 1, 169–173.CrossRefGoogle Scholar
  9. Gottesman S. (1984). Annu. Rev. Genetics. 18, 415–441.CrossRefGoogle Scholar
  10. Guzzo J., Cavin J.F. and Divies C. (1994). Biotechnol. Letters 16, 1189–1194.CrossRefGoogle Scholar
  11. Hall B.G. (1983) J. Bacteriol. 156, 1363–1365.PubMedGoogle Scholar
  12. Hecker M. and Völker U. (1990). FEMS Microbiol. Ecol. 74, 197–214.CrossRefGoogle Scholar
  13. Ingram L. (1986). Trends Biotechnol. 4, 40–44.CrossRefGoogle Scholar
  14. Ingram L. and Buttke T. (1984). Adv. Microbial Physiol. 25, 253–300.CrossRefGoogle Scholar
  15. LaRossa A. and Van Dyk T.K. (1991). Mol. Microbiol. 5, 529–534.PubMedCrossRefGoogle Scholar
  16. Lindquist S. and Craig E.A. (1998). Annu. Rev. Genetics. 22, 631–677.CrossRefGoogle Scholar
  17. McCallum K.L. and Iniss W.E. (1990). Arch. Microbiol. 153, 585–590.CrossRefGoogle Scholar
  18. Neidhardt F.C., VanBogelen R.A. and Vaughn V. (1984). Annu. Rev. Genetics. 18, 295–329.CrossRefGoogle Scholar
  19. Sanchez Y., Taulien J., Borkovich K.A. and Lindquist S. (1992). The EMBO Journal 11, 2357–2364.PubMedGoogle Scholar
  20. Völker U., Mach H., Schmid R. and Hecker M. (1992). J. Gen. Microbiol. 138, 2125–2135.PubMedGoogle Scholar
  21. Watson K., Dunlop G. and Cavicchioli R. (1984). FEBS Letters. 169, 267–173.CrossRefGoogle Scholar
  22. Zeuthen M.L., Dabrowa N., Aniebo C.M. and Howard D.H. (1988). J. Gen. Microbiol. 134, 1375–1384.PubMedGoogle Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • José António Couto
    • 1
  • Cristina Pina
    • 1
  • Tim Hogg
    • 1
  1. 1.Escola Superior de BiotecnologiaUniversidade Católica Portuguesa, R. A. Bernardino AlmeidaPortoPortugal

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