Biotechnology Letters

, Volume 19, Issue 5, pp 487–490

Enhancement of apparent resistance to ethanol in Lactobacillus hilgardii

  • José António Couto
  • Cristina Pina
  • Tim Hogg


The survival of Lactobacillus hilgardii, a highly ethanol-tolerant organism, after an ethanol challenge at 25% (v/v) for 10 min, increased by several log cycles when cells, grown in the absence of ethanol, were pre-treated with 10% (v/v) ethanol, 15% (v/v) methanol or 2% (v/v) butanol for 4 h. A temperature upshift (25 to 40°C) before ethanol challenge demonstrated a similar enhancement of apparent resistance to ethanol. Ethanol shock enhanced apparent resistance to methanol, butanol and heat challenges. The addition of chloramphenicol to cells prior to any pre-treatment did not significantly diminish the increase in ethanol tolerance, suggesting that de novo protein synthesis is not required for induced tolerance in this organism.


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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • José António Couto
    • 1
  • Cristina Pina
    • 1
  • Tim Hogg
    • 1
  1. 1.Escola Superior de BiotecnologiaUniversidade Católica Portuguesa, R. A. Bernardino AlmeidaPortoPortugal

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