Euphytica

, Volume 102, Issue 1, pp 9–13 | Cite as

A molecular and embryonic study of apomixis in cassava (Manihot esculenta Crantz)

  • Nagib M.A. Nassar
  • Marco Andre Vieira
  • Clibas Vieira
  • Dario Grattapaglia
Article

Abstract

In cassava, apomixis fixes heterosis and avoids transmission of systemic pathogens which complicate vegetative propagation of the crop. A combination of evidence from maternal inheritance of RAPD markers and the structure of the embryonic sac in large progeny sets of two distinct genotypes have further confirmed the occurrence of apomixis in cassava. We could advance further on earlier reports of the detection of apomixis in four ways: (1) we could arrive at an estimate of the rate of facultative apomixis in the range of 2%; (2) we detected the occurrence of apomixis in a second genotype, derived from a different interspecific cross; (3) apomictic behavior was demonstrated in an F1 individual and (4) parallel embryonic evidence was generated that corroborate the potential occurrence of apomixis by apospory. The fact that apomixis was detected in an F1 interspecific hybrid hints to the possibility of directly transferring genes for apomixis from a wild relative to cultivated cassava.

embryo sac RAPD interspecific hybrid 

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References

  1. Asker, S., 1979. Progress in apomixis research. Hereditas 91: 231- 240.Google Scholar
  2. Asker, S., 1980. Gametophytic apomixis:elements and genetic regulation. Hereditas 93: 277-293.CrossRefGoogle Scholar
  3. Doyle, J.J. & J.L. Doyle, 1987. Isolation of plant DNA from fresh tissues. Focus 12: 13-15.Google Scholar
  4. FAO, 1994. FAO Yearbook. Rome.Google Scholar
  5. Grattapaglia, D. & R.R. Sederoff, 1994. Genetic linkage maps of Eucalyptus grandisand E. urophyllausing a pseudotestcross strategy and RAPD markers. Genetics 137: 1121-1137, 1994PubMedGoogle Scholar
  6. Grattapaglia, D., C. Costa e Silva & N.M.A. Nassar, 1996. Strict maternal inheritance of RAPD fingerprints confirm apomixis in cassava (Manihot esculentaCrantz). Can J Plant Sci 76: 379-382.Google Scholar
  7. Herr, J.M., Jr., 1982. An analysis of methods for permanently mounting ovules cleared in four-and-a-half type clearing fluids. Stain Technol 57: 161-169.PubMedGoogle Scholar
  8. Nassar, N.M.A., 1980. Attempts to hybridize wild Manihotspecies with cassava. Econ Bot 34: 13-15.Google Scholar
  9. Nassar, N.M.A., 1994. Development and selection of apomixis in cassava, Manihot esculentaCrantz. Can J Plant Sci 74: 857-858.Google Scholar
  10. Novy, R.G., C. Kobak, J. Gofreda & N. Vorsa, 1994. RAPDs identify varietal misclassification and regional divergence in cranberry (Vaccinium macrocarpon(Ait.) Pursh). Theor Appl Genet 88: 1004-1010.CrossRefGoogle Scholar
  11. Ogburia, M.N. & T. Adachi, 1994. An improved cleared-pistil technique for rapid in totoobservation of embryo sac malformation in cassava (Manihot esculentaCrantz). Proceedings of the Second International Scientific Meeting Cassava Biotechnology Network, Indonesia, pp. 117-127.Google Scholar
  12. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531- 6535.PubMedGoogle Scholar
  13. Young, B.A., R.T. Sherwood & E.C. Bashaw, 1979. Cleared-pistil and thicksectioning techniques for detecting aposporous apomixis in grasses. Can J Bot 57: 1668-1672.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Nagib M.A. Nassar
    • 1
  • Marco Andre Vieira
    • 1
  • Clibas Vieira
    • 2
  • Dario Grattapaglia
    • 3
  1. 1.Departamento de AgronomiaUniversidade de BrasíliaBrasília D.F
  2. 2.Departamento de FitotecniaUniversidade de VicosaVicosaBrasília
  3. 3.Laboratório de Genética de Plantas CENARGEN-EMBRAPA –Brasília D.F

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