Biologia Plantarum

, Volume 44, Issue 2, pp 161–165 | Cite as

Insertion Profiles in Stomatal Density and Sizes in Nicotiana Tabacum L. Plantlets

  • M. Voleníková
  • I. Tichá


Tobacco (Nicotiana tabacum L. cv. Samsun) plantlets were cultured in vitro on Murashige-Skoog medium photoautotrophically (without sucrose) or photomixotrophically (with 3 % sucrose) under two irradiances [70 or 230 µmol m−2 s−1]. Significant differences in stomatal density and sizes in leaves of different insertion levels (3rd, 5th and 7th leaves from bottom) in photomixotrophic plantlets but not in photoautotrophic ones were found after 35 d of culture. Stomatal density was higher in upper leaves and on abaxial leaf side. Higher irradiance enhanced stomatal density in photoautotrophic plantlets. Stomatal sizes decreased with leaf insertion level but no significant differences between leaf sides were found. Abaxial stomata were more circular than the adaxial ones. In photomixotrophic plantlets stomata tended to be more elongated in the 3rd and the 5th leaves, whereas stomatal elongation in photoautotrophic plantlets was similar in all leaves.

irradiance photoautotrophic and photomixotrophic growth plant heterogeneity stomatal elongation and shape sucrose 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • M. Voleníková
    • 1
  • I. Tichá
    • 2
  1. 1.Faculty of Science, Department of Plant PhysiologyCharles University of PraguePraha 2Czech Republic
  2. 2.Faculty of Science, Department of Plant PhysiologyCharles University of PraguePraha 2Czech Republic

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