Molecular and Cellular Biochemistry

, Volume 229, Issue 1–2, pp 19–23 | Cite as

Inhibition of human low density lipoprotein oxidation by active principles from spices

  • K. Akhilender Naidu
  • N.B. Thippeswamy


Spice components and their active principles are potential antioxidants. In this study we examined the effect of phenolic and non-phenolic active principles of common spices on copper ion-induced lipid peroxidation of human low density lipoprotein (LDL) by measuring the formation of thiobarbituric acid reactive substance (TBARS) and relative electrophoretic mobility (REM) of LDL on agarose gel. Curcurriin, capsaicin, quercetin, piperine, eugenol and allyl sulfide inhibited the formation of TBARS effectively through out the incubation period of 12 h and decreased the REM of LDL. Spice phenolic active principles viz. curcumin, quercetin and capsaicin at 10 μM produced 40–85% inhibition of LDL oxidation at different time intervals while non-phenolic antioxidant allyl sulfide was less potent in inhibiting oxidation of LDL. However, allyl sulfide, eugenol and ascorbic acid showed pro-oxidant activity at lower concentrations (10 μM) and antioxidant activity at higher concentrations (50 μM) only. Among the spice principles tested quercetin and curcumin showed the highest inhibitory activity while piperine showed least antioxidant activity at equimolar concentration during initiation phase of oxidation of LDL. The inhibitory effect of curcumin, quercetin and capsaicin was comparable to that of BHA, but relatively more potent than ascorbic acid. Further, the effect of curcurnin, quercetin, capsaicin and BHA on initiation and propagation phases of LDL oxidation showed that curcurnin significantly inhibited both initiation and propagation phases of LDL oxidation, while quercetin was found to be ineffective at propagation phase. These data suggest that the above spice active principles, which constitute about 1–4% of above spices, are effective antioxidants and offer protection against oxidation of human LDL.

LDL oxidation curcumin quercetin capsaicin TBARS protective effect spice active principles 


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  1. 1.
    Hessler JR., Morel DW, Lewis LJ, Chisolm GM: Lipoprotein oxidation and lipoprotein-induced cytotoxicity. Atherosclerosis 3: 215–222, 1983Google Scholar
  2. 2.
    Dimmeler S, Haendeler J, Galle J, Zeiher AM: Oxidized low-density lipoprotein induces apoptosis of human endothelial cells by activation of CPP32-like proteases. A mechanistic clue to the ‘response to injury’ hypothesis. Circulation 95: 1760–1763, 1997Google Scholar
  3. 3.
    Nishio F, Watanabe Y: Oxysterols induce apoptosis in cultured smooth muscle cells through CPP32 protease activation and bcl-2 protein down regulation. Biochem Biophys Res Commun 226: 928–934, 1996Google Scholar
  4. 4.
    Escargueil-Blanc I, Salvayre R, Negre-Salvayre A: Necrosis and apoptosis induced by oxidized low density lipoproteins occurs through calcium-dependent pathway in lymphoblastoid cells. FASEB J 8: 1075–1080, 1994Google Scholar
  5. 5.
    Papassotiropoulos A, Ludwig M, Naib-Majani W, Rao GS: Induction of apoptosis and secondary necrosis in rat dorsal root ganglion cell cultures by oxidized low density lipoprotein. Neuro Lett 209: 33–36, 1996Google Scholar
  6. 6.
    Sato K, Niki E, Shimasaki H: Free radical mediated chain oxidation of low density lipoprotein and its synergistic inhibition by vitamin E and vitamin C. Arch Biochem Biophys 279: 402–405, 1990Google Scholar
  7. 7.
    Parthasarthy S, Young SG, Witztum JL, Pittman RC, Steinberg DS: Probucol inhibits oxidative modification of low density lipoprotein. J Clin Invest 77: 641–644, 1991Google Scholar
  8. 8.
    Esterbauer H, Striegl G, Puhl H, Oberreither S, Rotheneder M, el-Saadani M, Jurgens G: The role of vitamin E and carotenoids in preventing oxidation of low density lipoproteins. Ann NY Acad Sci 570: 254–267 1989Google Scholar
  9. 9.
    Frankel EN, Kanner J, German JB, Parks E, Kinsella JE: Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 341: 454–547, 1993Google Scholar
  10. 10.
    Miyagi Y, Miwa K, Inoue H: Inhibition of low-density lipoprotein oxidation by flavonoids in red wine and grape juice. Am J Cardiol 80: 1627–1631, 1997Google Scholar
  11. 11.
    Nigdikar SV, Williams NR, Griffin BA, Howard AN: Consumption of red wine polyphenols reduce the susceptibility of low-density lipoproteins to oxidation in vivo. Am J Clin Nutr 68: 258–265, 1998Google Scholar
  12. 12.
    Schuh J, Fariclough GF, Haschemeyer RH: Oxygen mediated heterogeneity of apo-low density lipoprotein. Proc Natl Acad Sci USA 75: 3173–3177, 1978Google Scholar
  13. 13.
    Noble RP: Electrophoretic separation of plasma lipoproteins in agarose gel. J Lipid Res 9: 693–700, 1968Google Scholar
  14. 14.
    Pulla Reddy Ach, Lokesh BR: Studies on spice principles as antioxidants in the inhibition of lipid peroxidation of rat liver microsomes. Mol Cell Biochem 111: 117–124, 1992Google Scholar
  15. 15.
    Naidu KA: Eugenol — an inhibitor of lipoxygenase-dependent lipid peroxidation. Prostagland Leukot Essent Fatty Acids 53: 381–384, 1995Google Scholar
  16. 16.
    Brown JE, Khodr H, Hider RC, Rice-Evans CA: Structural dependence of flavonoid interaction with Cu2+ ions: Implications for their antioxidant properties. Biochem J 330: 1173–1178, 1998Google Scholar
  17. 17.
    Ramirez-Tortosa MC, Meas MD, Aguilera MC, Quiles JL, Baro L, Rarnirez-Tortosa CL, Martinez-Victoria E, Gil A: Oral administration of turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atherosclerosis 147: 371–380,1999Google Scholar
  18. 18.
    Luiz da Silva E, Tsushida T, Terao J: Inhibition of mammalian 15-lipoxygenase-dependent lipid peroxidation in low-density lipoprotein by quercetin and quercetin monoglucosides. Arch Biochem Biophys 349: 313–320, 1998Google Scholar
  19. 19.
    Phelps S, Harris WS: Garlic supplementation and lipoprotein oxidation susceptibility. Lipids 28: 475–477, 1993Google Scholar
  20. 20.
    Munday JS, James KA, Fray LM, Kirkwood SW, Thompson KG: Daily supplementation with aged garlic extract, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis 143: 399–404, 1999Google Scholar
  21. 21.
    Fuhrman B, Rosenblat M, Hayek T, Coleman R, Avirain M: Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in apolipoprotein E-deficient mice. J Nutr 130: 1124–1131, 2000Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Department of Biochemistry and NutritionCentral Food Technological Research InstituteMysoreIndia

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