Journal of Applied Phycology

, Volume 13, Issue 4, pp 335–342 | Cite as

Molecular differentiation of two morphological variants of Gracilaria salicornia

  • Phaik-Eem Lim
  • Kwai-Lin Thong
  • Siew-Moi Phang
Article

Abstract

Xia in 1986 combined Gracilaria salicornia, G. canaliculata (G. crassa), G. cacalia and G. minor into one species: G.salicornia. Two morphological variants of G. salicornia were collectedfrom different localities in Malaysia. Variant A collected from Morib,Selangor grew on the roots of Avicennia. The samples showed absenceof main axis; segmented constrictions throughout; cylindrical or slightlycompressed thalli. Variant B was collected from the mudflats of TanjungTuan, growing on rocks, coral or forming mats on the mud. Plants showedabsence of main axis; segments were not constricted throughout the plant(if present only slightly articulated at the upper part), branching wasdichotomous or irregular; cylindrical or slightly compressed thalli. Thetechnique of Random Amplified Polymorphic DNA analysis (RAPD) wasused to investigate molecular characteristics of the two variants. Out ofsixty Operon primers that were screened, four primers, OPA 1, OPA 10,OPA 11 and OPK 7 were able to give polymorphism. The fingerprintsgenerated were stable and reproducible on repeated analysis. The DNAfingerprints generated were visually analysed and clustering analysis wascarried out using GelCompar 4.0. The matrix of similarities was based onthe Dice coefficients (SD) and the cluster analysis was carried outusing the unweighted pair group method using arithmetic averages(UPGMA). DNA analysis showed that two primers (OPA 01, CAGGCCCTTC and OPK 07, AGCGAGCAAG) were able to differentiate the two variants.

Gracilaria salicornia molecular taxonomy random amplified polymorphic DNA (RAPD) 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Phaik-Eem Lim
    • 1
  • Kwai-Lin Thong
    • 1
  • Siew-Moi Phang
    • 1
  1. 1.Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia

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