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World Journal of Microbiology and Biotechnology

, Volume 18, Issue 7, pp 627–636 | Cite as

Biological control of seedling blight of wheat caused by Fusarium graminearum with beneficial rhizosphere microorganisms

  • G.M. Dal Bello
  • C.I. Mónaco
  • M.R. Simón
Article

Abstract

Fusarium graminearum is associated with the cereal damping-off complex which reduces germination, seedling stand and yield. Fifty-two bacterial strains and six Trichoderma spp. isolated from the wheat rhizosphere were evaluated for biocontrol of seedling blight of wheat caused by F. graminearum. Their potential as biocontrol agents was tested in vitro and in the greenhouse. Isolates varied in their ability to inhibit the mycelial growth of F. graminearum in agar plate bioassays by 0–79%. This parameter was not related with biocontrol efficacy of in vivo assays. In greenhouse trials, all isolates were initially evaluated for reducing disease on wheat cultivars Klein Centauro (moderately resistant to F. graminearum) and Pro INTA Oasis (susceptible) planted in sterilized soil artificially infested with the pathogen. Among the 25 bacteria and six fungal isolates that exhibited a pronounced suppressive effect, the most efficient 10 for both cultivars were further assayed on eight cultivars (Buck Candil, Buck Catriel, Buck Chambergo, Buck Poncho, Buck Topacio, Klein Cacique, Klein Centauro and Pro INTA Oasis) potted in cultivated–inoculated soil. Three weeks after sowing, plant stand, percentage of diseased emerging seedlings, plant height and dry weight were evaluated. Among the antagonists only Stenotrophomonas maltophilia was significantly better than the control for the average of the eight cultivars for plant stand, height and dry weight. Stenotrophomonas maltophilia also caused a non-significant decrease in the percentage of diseased plants. Three strains of Bacillus cereus and one isolate of Trichoderma harzianum gave also a good control in some cultivars. The ability of these isolates to affect the infection of wheat seedlings by F. graminearum may be of potential value in field trials.

Biocontrol Fusarium graminearum seedling blight wheat 

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References

  1. Alippi, A., Perelló, A., Sisterna, M., Greco, N. & Cordo, C. 2000 Potential of spore-forming bacteria as biocontrol agents of wheat foliar diseases under laboratory and greenhouse conditions. Journal of Plant Diseases and Protection 107, 155–169.Google Scholar
  2. Baird, R., Nankam, C., Moghaddam P. & Pataky, J. 1994 Evaluation of seed treatments on Shrunken-2 sweet corn. Plant Disease 78, 817–821.Google Scholar
  3. Bernard, P., Romond, Ch. & Bhatnagar, T. 1995 Biological control of Pythium mamillatum causing damping-off of cucumber seedlings by a soil bacterium, Bacillus mycoides. Microbiological Research 150, 71–75.Google Scholar
  4. Bharat, R. & Singh, D. 1980 Antagonistic activity of some leaf surface microfungi against Alternaria brassicae and Drechslera graminea. Transactions of the British Mycological Society 75, 363–369.Google Scholar
  5. Campbell, R. 1986 The search for biological control agents against plant pathogens: a pragmatic approach. Biological Agriculture and Horticulture 3, 317–327.Google Scholar
  6. Camporota, P. 1985 Antagonisme in vitro de Trichoderma spp. vis-à-vis de Rhizoctonia solani Kühn. Agronomie 5, 613–620.Google Scholar
  7. Cappellini, R & Peterson, J. 1965 Macroconidium formation in submerged cultures by a nonsporulating strain of Gibberella zeae. Plant Disease 66, 962–966.Google Scholar
  8. Chao, W., Nelson, E., Harman, G. & Hoch, H. 1986 Colonization of the rhizosphere by biological control agents applied to seeds. Phytopathology 76, 60–65.Google Scholar
  9. Cook, J. 1968 Fusarium root and foot rot of cereals in the Pacific Northwest. Phytopathology 58, 127–131.Google Scholar
  10. Cook, J. & Baker, K. 1983 The Nature and Practice of Biological Control of Plant Pathogens. St. Paul, MN: The American Phytopathological Society. ISBN 0-89054053-5.Google Scholar
  11. Daamen, R., Wijnands, F. & Vilet, G. van der 1989 Epidemics of diseases and pest of winter wheat at different levels of agrochemical imput. Journal of Phytopathology 125, 305–319.Google Scholar
  12. Dal Bello, G. 1982 Selección de dos medios de cultivo adaptados al aislamiento de hongos del suelo. In Proceedings 1982 2° Congreso Latinoamericano de Fitopatología. Buenos Aires, pp. 42–52.Google Scholar
  13. Datnoff, L.E., Nemec, S. & Pernezny, K. 1995 Biological control of Fusarium crown and root rot of tomato in Florida using Trichoderma harzianum and Glomus intraradices. Biological Control 5, 427–431.Google Scholar
  14. De Freitas, J. & Germida, J. 1991 Pseudomonas cepacia and Pseudomonas putida as winter wheat inoculants for biocontrol of Rhizoctonia solani. Canadian Journal of Microbiology 37, 780–784.Google Scholar
  15. Dunne, C., Crowley, J., Moenne-Loccoz, K., Dowling, D., de Bruijn, F. & O'Gara, F. 1997 Biological control of Pythium ultimum by Stenotrophomonas maltophilia W81 is mediated by an extracellular proteolytic activity. Microbiology 143, 3921–3931.Google Scholar
  16. Fokkema, N. 1973 The role of saprophytic fungi in antagonism against Drechslera sorokiniana (Helminthosporium sativum) on agar plates and on rye leaves with pollen. Physiological Plant Pathology 3, 195–205.Google Scholar
  17. Fravel, D.R. 1988 Role of antibiosis in the biocontrol of plant diseases. Annual Review of Phytopathology 26, 75–91.Google Scholar
  18. Giesler, L. & Yuen, G. 1998 Evaluation of Stenotrophomonas maltophilia strain C3 for biocontrol of brown patch disease. Crop Protection 17, 509–513.Google Scholar
  19. Handelsman, J., Raffel, S., Mester, E., Wunderlich, L. & Gray, C. 1990 Biological control of damping-off of alfalfa seedlings with Bacillus cereus UW 85. Applied and Environmental Microbiology 56, 713–718.Google Scholar
  20. Harman, G. 1992 Development and benefits of rhizosphere competent fungi for biological control of plant pathogens. Journal of Plant Nutrirtion 15, 835–843.Google Scholar
  21. Harman, A., Taylor, A. & Stasz, T. 1989 Combining effective strains of Trichoderma harzianum and solid matrix priming to improve biological seed treatments. Plant Disease 73, 631–637.Google Scholar
  22. Hedegus, A. & Farkas, S. 1997 Interaction of antagonistic microorganisms and Fusarium strains on root of seedlings of wheat cultivars. Cereal Research Communications 25, 749–751.Google Scholar
  23. Hering, T., Cook, R. & Tang, W. 1987 Infection of wheat embryos by Pythium species during seed germination and the influence of seed age and soil matric potential. Phytopathology 77, 1104–1108.Google Scholar
  24. Jackson, A.J., Walters, D. & Marshall, G. 1997 Antagonistic interactions between the foliar pathogen Botrytis fabae and isolates of Penicillium brevicompactum and Cladosporium cladosporioides on faba beans. Biological Control 8, 97–116.Google Scholar
  25. Kim, D., Cook, R. & Weller, D. 1997 Bacillus sp. L324-92 for biological control of three root diseases of wheat grown with reduced tillage. Phytopathology 87, 551–558.Google Scholar
  26. Kloepper, J. 1991 Development of in vivo assays for prescreening antagonists of Rhizoctonia solani on cotton. Phytopathology 81, 1006–1013.Google Scholar
  27. Knudsen, Y., Hockenhull, J. & Jensen, D. 1995 Biocontrol of seedling diseases of barley and wheat caused by Fusarium culmorum and Bipolaris sorokiniana: effects of selected fungal antagonists on growth and yield components. Plant Pathology 44, 467–477.Google Scholar
  28. Kong, G., Kochman, J. & Brow, J. 1997 Phylloplane bacteria antagonistic to the sunflower pathogen Alternaria helianthi Australas. Plant Pathology 26, 85–97.Google Scholar
  29. Kwok, O., Fahy, P., Hoitink, H. & Kuter, G. 1987 Interactions between bacteria and Trichoderma hamatum in suppression of Rhizoctonia damping-off in bark compost media. Phytopathology 77, 1206–1212.Google Scholar
  30. Lazzaretti, E., Menten, J. & Bettiol, W. 1994 Bacilus subtilis antagonistic to the principal pathogens associated with bean and wheat seeds. Fitopatología Venezolana 7, 42–46.Google Scholar
  31. Lewis, J. & Papavizas, G. 1991 Biocontrol of plant diseases: the approach for tomorrow. Crop Protection 10, 95–105.Google Scholar
  32. Liang, X., Huang, H., Yanke, L. & Kozub, G. 1996 Control of damping-off of saffower by bacterial seed treatment. Canadian Journal of Plant Pathology 18, 43–49.Google Scholar
  33. Lo, C., Nelson, E. & Harman, G. 1996 Biological control of turfgrass with a rhizosphere competent strain of Trichoderma harzianum. Plant Disease 80, 736–741.Google Scholar
  34. Luz, W. da., Bergstrom, G. & Stockwell, C. 1998 Seed-applied bioprotectants for control of seedborne Pyrenophora tritici-repentis and agronomic enhancement of wheat. Canadian Journal of Plant Pathology 19, 384–386.Google Scholar
  35. Mao, W., Lewis, J., Hebbar, P. & Lumsden, R. 1997 Seed treatment with a fungal or a bacterial antagonist for reducing corn damping-off caused by species of Pythium and Fusarium. Plant Diseae 81, 450–454.Google Scholar
  36. Mao, W., Lumsden, R., Lewis, J. & Hebbar, P. 1998 Seed treatment using pre-infiltration and biocontrol agents to reduce damping-off of corn by species of Pythium and Fusarium. Plant Disease 89, 294–299.Google Scholar
  37. Mathre, D., Johnston, R., Callan, N., Mohan, S., Martin, J. & Miller, J. 1995. Combined biological and chemical seed treatments for control of two seedling diseases of Sh2 sweet corn. Plant Disease 79, 1145–1148.Google Scholar
  38. Radulescu, E. & Negru, A. 1965 Contribution to the study of the mycoflora of the seed coat of germinating seeds. Probleme Agriculturale 17, 37–42.Google Scholar
  39. Silo-Suh, L., Lethbridge, B., Raffel, S., He, H., Clardy, J. & Handelsman, J. 1994 Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW 85. Applied and Environmental Microbiology 60, 2023–2030.Google Scholar
  40. Spencer, R. 1995 Emergence of epidemic multiple antibiotic resistant Stenotrophomonas (Xanthomonas) maltophilia and Burkholderia (Pseudomona) cepacia. Journal of Hospital Infection 30, 453–464.Google Scholar
  41. Sutton, J. 1982 Epidemiology of wheat head blight maize ear rot caused by Fusariumgram inearum. Canadian Journal of Plant Pathology 4, 195–209.Google Scholar
  42. Swadling, I. & Jeffries, P. 1996 Isolation of microbial antagonists for biocontrol of grey mould diseases of strawberries. Biocontrol Science and Technology 6, 125–136.Google Scholar
  43. Webber, J. & Hedger, J. 1986 Comparison of interactions between Ceratocystis ulmi and elm bark saprobes in vitro and in vivo. Transactions of the British Mycological Society 86, 93–101.Google Scholar
  44. Zhang, Z. & Yuen, G. 1999 Biological control of Bipolaris sorokiniana on tall fescue by Stenotrophomonas maltophilia C3. Phytopathology 89, 817–822.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • G.M. Dal Bello
    • 1
  • C.I. Mónaco
    • 1
  • M.R. Simón
    • 2
  1. 1.Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y ForestalesUniversidad Nacional de La Plata, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC)La Plata, Buenos AiresArgentina
  2. 2.Cerealicultura, Facultad de Ciencias Agrarias y ForestalesUniversidad Nacional de La PlataLa Plata, Buenos AiresArgentina

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