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Journal of Fluorescence

, Volume 12, Issue 2, pp 173–175 | Cite as

New Approach for Fluorescent Peptide Labeling with Pyrylium Cyanine Dyes

  • O. M. Kostenko
  • S. Y. Dmitrieva
  • O. I. Tolmachev
  • S. M. Yarmoluk
Article

Abstract

New approach for fluorescent peptide labeling with cyanine dyes utilizing the reaction of pyrylium salts with aliphatic aminogroup is proposed. The reaction of two pyrylium cyanines dye acids was investigated. Lysocyme was used as a model peptide for conjugation. The proposed method can be used as a simple and convenient alternative for the known procedures because it does not require preparation of the unstable amino-reactive intermediates from a carboxyl- or sulfo-derivative of cyanine dyes.

Cyanine dyes peptide labeling fluorescent detection 

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REFERENCE

  1. 1.
    S. Yarmoluk, O. Kostenko, and I. Dubey (2000) Bioorga. Medicinal Genetics, Chem. Lett., 10, 2201–2204.Google Scholar

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • O. M. Kostenko
    • 1
  • S. Y. Dmitrieva
    • 1
  • O. I. Tolmachev
    • 2
  • S. M. Yarmoluk
    • 1
  1. 1.Institute of Molecular Biology and Genetics NAS of UkraineKyivUkraine
  2. 2.Institute of Organic Chemistry, NAS of UkraineKyivUkraine

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