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World Journal of Microbiology and Biotechnology

, Volume 18, Issue 7, pp 637–644 | Cite as

The effects of the novel bifidogenic trisaccharide, neokestose, on the human colonic microbiota

  • Stephanus Kilian
  • Susanna Kritzinger
  • Catherine Rycroft
  • Glenn Gibson
  • James du Preez
Article

Abstract

The potential prebiotic effect of the fructo-trisaccharide, neokestose, on intestinal bacteria was investigated. Bifidobacterium sp. utilized neokestose to a greater extend and produced more biomass from neokestose than facultative anaerobes under anaerobic conditions in batch culture. Lactobacillus salivarius utilized glucose but negligible amounts of neokestose. L. salivarius and the facultative anaerobes produced significantly more biomass from glucose than from neokestose, whereas the biomass yields obtained with bifidobacteria on neokestose and glucose, respectively, were not significantly different. Static batch cultures inoculated with faeces supported the prebiotic effect of neokestose, which had been observed in the pure culture investigations. Bifidobacteria and lactobacilli were increased while potentially detrimental coliforms, clostridia and bacteroides, decreased after 24 h fermentation with neokestose. In addition, this effect was more pronounced with neokestose than with a commercial prebiotic fructo-oligosaccharide. It was concluded that neokestose has potential as a novel bifidogenic substance and that it might have advantages over the commercially available sources currently used.

Bifidogenic neokestose oligosaccharide Phaffia prebiotic Xanthophyllomyces 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Stephanus Kilian
    • 1
  • Susanna Kritzinger
    • 1
  • Catherine Rycroft
    • 2
  • Glenn Gibson
    • 2
  • James du Preez
    • 1
  1. 1.Department of Microbiology and BiochemistryUniversity of the Free StateBloemfonteinSouth Africa
  2. 2.Food Microbial Sciences Unit, Department of Food Science and TechnologyThe University of Reading, WhiteknightsReadingUK

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