Physiology: DAZLA: An Important Candidate Gene in Male Subfertility?

  • Ron J. T. van Golde
  • Joep H. A. M. Tuerlings
  • Jan A. M. Kremer
  • Didi D. M. Braat
  • Frans Schoute
  • Lies H. Hoefsloot


Purpose: To study the role of the autosomal candidate gene DAZLA (Deleted in AZoospermia Like Autosome) in male subfertility.

Methods: We reviewed clinical data of subfertile men with oligozoospermia or azoospermia, mostly candidates for intracytoplasmic sperm injection (ICSI). Mutation detection was performed using polymerase chain reaction followed by single strand conformation polymorphism analysis. All shifted bands were analyzed by sequencing.

Results: We searched for mutations in 44 subfertile men. Nine subfertile men were included, because family history showed that their brothers also faced fertility problems. In these men a possible autosomal gene defect may contribute to their fertility problem. No mutations were found, except for two polymorphisms in intron 4 and 5.

Conclusion: At this moment it does not seem relevant to search for possible mutations in the DAZLA gene in clinical practice.

DAZLA genetics ICSI male subfertility 


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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Ron J. T. van Golde
    • 1
  • Joep H. A. M. Tuerlings
    • 2
  • Jan A. M. Kremer
    • 1
  • Didi D. M. Braat
    • 1
  • Frans Schoute
    • 2
  • Lies H. Hoefsloot
    • 2
  1. 1.Department of Obstetrics and GynecologyUniversity Medical Center NijmegenNijmegenThe Netherlands
  2. 2.Department of Human GeneticsUniversity Medical Center NijmegenNijmegenThe Netherlands

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