Journal of Assisted Reproduction and Genetics

, Volume 18, Issue 8, pp 407–412

Andrology: Follicular Fluid Enhances Sperm Attraction and Its Motility in Human

  • Byeong-Gyun Jeon
  • Jin-Soo Moon
  • Kwang-Cheul Kim
  • Hyo-Jong Lee
  • Sang-Yong Choe
  • Gyu-Jin Rho
Article

Abstract

Purpose: Follicular fluid has a pivotal effect on motility and chemotaxis of spermatozoa for successful fertilization. The effect of human follicular fluid (hFF) and progesterone on attraction and motility of spermatozoa were investigated using simplified capillary assays.

Methods: Capillary tubes loaded with hFF, modified human tubal fluid (m-hTF), or m-hTF supplemented with progesterone, respectively, were used for assessments of attraction and motility of spermatozoa following culture at various time intervals.

Results: Number and motile ratio of spermatozoa in the tubes loaded with hFF were significantly (P < .05) higher than those with m-hTF. In the tubes loaded with m-hTF, m-hTF supplemented with progesterone, and hFF, the attracted number of spermatozoa were 34 × 105, 131 × 105, and 108 × 105, and motile ratio of spermatozoa was 37, 48, and 82%, respectively.

Conclusions: We conclude that hFF clearly plays a crucial role in enhancing attraction and motility of spermatozoa, and progesterone has strong effect on attraction of spermatozoa.

attraction follicular fluid human motility spermatozoa 

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REFERENCES

  1. 1.
    Burkman LJ: Hyperactivated motility of human spermatozoa during in vitro capacitation and implications for fertility. InControls of Sperm Motility: Biological and Clinical Aspects, C Gagno (ed), Boca Raton, CRC press, 1990, pp 303-329Google Scholar
  2. 2.
    Chao HT, Ng HT, Kao SH, Wei YH, Hong CY: Human follicular fluid stimulates the motility of washed human sperm. Arch Androl 1991;26:61-65Google Scholar
  3. 3.
    Brucker C, Lipford GB: The human sperm acrosome reaction: Physiology and regulatory mechanisms. Hum Reprod Update 1995;1:51-62Google Scholar
  4. 4.
    Hernandez Alvarado SR, Guzman-Grenfell AM, Hicks Gomez JJ: Communication of gamete at distance. Chemotaxis and chemokinesis in spermatozoa in mammals. Gynecol Obstet Mex 1995;63:323-327Google Scholar
  5. 5.
    Ito M, Smitt TT, Yanagimachi R: Effect of ovulation on sperm transport in the hamster oviduct. J Reprod Fertil 1991;93:157-163Google Scholar
  6. 6.
    Hunter RHF, Wilmut I: Sperm transport in the cow: Periovulatory distribution of viable cells within the oviduct. Reprod Nutr Dev 1984;24:597-608Google Scholar
  7. 7.
    Hunter RHF, Nichol R: Transport of spermatozoa in the sheep in the ovuduct: Preovulatory sequestering of cells in the caudal isthmus. J Exp Zool 1983;228:121-128Google Scholar
  8. 8.
    Ralt D, Goldenberg M, Fetterolf P, Thomson D, Dor J, Mashiach S, Garbers DL, Eisenbach M: Sperm attraction to a follicular factor(s) correlates with human egg fertilizability. Proc Nat Acad Sci 1991;88:2840-2844Google Scholar
  9. 9.
    Williams M, Hill CJ, Scudamore I, Dunphy B, Cooke ID, Barratt CLR: Sperm numbers and distribution within the human fallopian tube around ovulation. Hum Reprod 1993;8:2019-2026Google Scholar
  10. 10.
    Fabbri R, Porcu E, Lenzi A, Gandini L, Marsella T, Flamigni C: Follicular fluid and human granulosa cell cultures: Influence on sperm kinetics parameters, hyperactivation and acrosome reaction. Fertil Steril 1998;69:112-117Google Scholar
  11. 11.
    Fetterolf PM, Sutherland CS, Josephy PD, Casper RF, Tyson JE: Preliminary characterization of a factor in human follicular fluid that stimulates human spermatozoa motion. Hum Reprod 1994;9:1505-1511Google Scholar
  12. 12.
    Kulin S, Bastians BA, Hollanders HMG, Janssen HJG, Goverde HJM: Human serum and follicular fluid stimulate hyperactivation of human spermatozoa after preincubation. Fertil Steril 1994;62:1234-1237Google Scholar
  13. 13.
    Mbizvo MT, Burman LJ, Alexander NJ: Standard conditions for a hyperactivation (HA) assay. Submitted for the annual meeting of the American fertility society, Washington DC, 1990Google Scholar
  14. 14.
    McNutt TL, Killian GJ: Influence of bovine follicular and oviduct fluids on sperm capacitation in vitro. J Androl 1991;12: 244-252Google Scholar
  15. 15.
    Mendoza C, Tesarik J: Effect of follicular fluid on sperm movement characteristics. Fertil Steril 1990;54:1135-1139Google Scholar
  16. 16.
    Ralt D, Manor M, Cohen-Dayag A, Tur-Kaspa I, Ben-Shlomo I, Makler A, Yuli I, Dor J, Blunberg S, Mashiach S, Eisenbach M: Chemotaxis and chemokinesis of human spermatozoa to follicular factors. Biol Reprod 1994;50:774-785Google Scholar
  17. 17.
    Krausz CS, Gervasi G, Forti G, Baldi E: Effect of platelet-activating factor on motility and acrosome reaction of human spermatozoa. Hum Reprod 1994;9:471-476Google Scholar
  18. 18.
    Ricker DD, Minhas BS, Krumar R, Robertson JL, Dodson MG: The effects of platelet activating factor on the motility of human spermatozoa. Fertil Steril 1989;52:655-658Google Scholar
  19. 19.
    Baldi E, Casano R, Falsetti C, Kruasz C, Maggi M, Forti G: Non-genomic actions of progesterone on human spermatozoa. Trends Endocrinol Metab 1995;6:198-205Google Scholar
  20. 20.
    Blackmore PF, Beebe SJ, Danforth DR, Alexander N: Progesterone and 17 alpha-hydroxyprogesterone. Novel stimulators of calcium influx in human sperm. J Biol Chem 1990;265:1376-1380Google Scholar
  21. 21.
    Cohen-Dayag A, Tur-Kaspa, Dor J, Mashiach S, Eisenbach M: Sperm capacitation in humans is transient and correlates with chemotactic responsiveness to follicular factors. Proc Natl Acad Sci USA 1995;11039-11043Google Scholar
  22. 22.
    Eisenbach M, Tur-Kaspa I: Human sperm chemotaxis is not enigmatic anymore. Fertil Steril 1994;62:233-235Google Scholar
  23. 23.
    Eisenbach M: Sperm chemotaxis. Rev Reprod 1999;4:56-66Google Scholar
  24. 24.
    Jaiswal BS, Tur-Kaspa I, Dor J, Mashiach S, Eisenbach M: Human sperm chemotaxis; Is progesterone chemoattractant? Biol Reprod 1999;60:1314-1319Google Scholar
  25. 25.
    Quinn P: Enhanced results in mouse and human embryo culture using a modified human tubal fluid medium lacking glucose and phosphate. J Asssist Reprod Genet 1995;12:97-105Google Scholar
  26. 26.
    Villanueva Diaz C, Arias Martinez J, Bermejo Martinez L, Vadillo Ortega F: Progesterone induces human sperm chemotaxis. Fertil Steril 1995;64:1183-1188Google Scholar
  27. 27.
    Sliwa L: Effect of some sex steroid hormones on human spermatozoa migration in vitro. Eur J Obstet Gynecol Reprod Biol 1995;58:173-175Google Scholar
  28. 28.
    World Health Organization: Laboratory for the examination of human serum and semen-cervical mucus interaction. Cambridge, Cambridge University Press, 1993.Google Scholar
  29. 29.
    Tesarik J, Mendoza C: Defective function of a nongenomic progesterone receptor as a sole sperm anomaly in infertile patients. Fertil Steril 1992;58:593-597Google Scholar
  30. 30.
    Eisenbach M, Tur-Kaspa I: Do human eggs attract spermatozoa? BioEssays 1999;21:203-210Google Scholar
  31. 31.
    Garbers DL: Identification of a cell surface receptor common to germ and somatic cells. Biol Reprod 1991;44:225-230Google Scholar
  32. 32.
    Carver-Ward JA, Hollanders JMG, Jaroudi KA, Einspenner M, Al-Sedairy ST, Sheth KV: Progesterone does not potentialize the acrosome reaction in human spermatozoa: Flow cytometric analysis using CD46 antibody. Hum Reprod 1996;11:121-126Google Scholar
  33. 33.
    Oehningher S, Blackmore PF, Morshedi M, Sueldo C, Acosta AA, Alexander NJ: Defective calcium influx and acrosome reaction (spontaneous and progesterone induced) in spermatozoa of infertile men with severe teratozoospermia. Fertil Steril 1994;61:349-354Google Scholar
  34. 34.
    Ravnik SE, Zarutskie PW, Muller CH: Lipid transfer activity in human follicular fluid relation to human capacitation. J Androl 1990;11:216-226Google Scholar
  35. 35.
    Ijaz A, Lambert RD, Sirard M: In vitro cultured bovine granulosa and oviductal cells secrete sperm motility-maintaining factor(s). Mol Reprod Dev 1994;37:54-60Google Scholar

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Byeong-Gyun Jeon
    • 1
  • Jin-Soo Moon
    • 1
  • Kwang-Cheul Kim
    • 1
  • Hyo-Jong Lee
    • 2
  • Sang-Yong Choe
    • 2
  • Gyu-Jin Rho
    • 2
  1. 1.Kaya Mother-Child's HospitalChinjuSouth Korea
  2. 2.College of Veterinary MedicineGyeongsang National UniversityChinjuSouth Korea

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