Journal of Fluorescence

, Volume 11, Issue 2, pp 79–87 | Cite as

Spectral Properties, Cation Selectivity, and Dynamic Efficiency of Fluorescent Alkali Ion Indicators in Aqueous Solution Around Neutral pH

  • Pierre Buet
  • Bernd Gersch
  • Ernst GrellEmail author


The spectral properties of two fluorescent alkali ion indicators, the commercially available cryptand CD222 and a new bipyridyl-type cryptand, F[bpy.bpy.2], bearing the trifluorocoumarino residue are investigated in aqueous solution as a function of pH as well as around neutral pH in the presence of alkali and alkaline earth cations. From the values of the acidity constants it is concluded that bridgehead nitrogen deprotonation occurs at a much lower pH for CD222 (pKa below 5.5) than for F[bpy.bpy.2]. Spectrofluorometric titrations with salts of NH+4, TI+, and alkali as well as alkaline earth cations indicate that both indicators are K+ selective. F[bpy.bpy.2] shows the higher K+/Na+ selectivity and larger fluorescence intensity changes but the slower dynamic response. Under suitable conditions, alkali ion binding by CD222 can occur in less than 1 ms.

Alkali ion indicator CD222 fluorescent cryptands stability constants dynamics of cation binding 


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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  1. 1.Max-Planck-Institute of BiophysicsFrankfurtGermany

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