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Journal of Fluorescence

, Volume 11, Issue 2, pp 79–87 | Cite as

Spectral Properties, Cation Selectivity, and Dynamic Efficiency of Fluorescent Alkali Ion Indicators in Aqueous Solution Around Neutral pH

  • Pierre Buet
  • Bernd Gersch
  • Ernst Grell
Article

Abstract

The spectral properties of two fluorescent alkali ion indicators, the commercially available cryptand CD222 and a new bipyridyl-type cryptand, F[bpy.bpy.2], bearing the trifluorocoumarino residue are investigated in aqueous solution as a function of pH as well as around neutral pH in the presence of alkali and alkaline earth cations. From the values of the acidity constants it is concluded that bridgehead nitrogen deprotonation occurs at a much lower pH for CD222 (pKa below 5.5) than for F[bpy.bpy.2]. Spectrofluorometric titrations with salts of NH+4, TI+, and alkali as well as alkaline earth cations indicate that both indicators are K+ selective. F[bpy.bpy.2] shows the higher K+/Na+ selectivity and larger fluorescence intensity changes but the slower dynamic response. Under suitable conditions, alkali ion binding by CD222 can occur in less than 1 ms.

Alkali ion indicator CD222 fluorescent cryptands stability constants dynamics of cation binding 

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REFERENCES

  1. 1.
    C. Krause, T. Werner, C. Huber, and O. S. Wolfbeis (1999) Anal. Chem. 71, 5304-5308.Google Scholar
  2. 2.
    R. Y. Tsien (1980) Biochemistry 19, 2396-2404.Google Scholar
  3. 3.
    B. Dietrich, J. M. Lehn, and J. P. Sauvage (1969) Tetrahedron Lett. 34, 2885-2888.Google Scholar
  4. 4.
    A. Minta and R. Y. Tsien (1989) J. Biol. Chem. 32, 19449-19457.Google Scholar
  5. 5.
    R. Crossley, Z. Goolamali, and P. G. Sammes (1994) J. Chem. Soc. Perkin Trans. 2, 1615-1623.Google Scholar
  6. 6.
    P. Buet, F. Kastenholz, E. Grell, G. Käb, A. Häfner, and F. W. Schneider (1999) J. Phys. Chem. 103, 5871-5881.Google Scholar
  7. 7.
    F. Kastenholz (1993) Ph.D. thesis, Max-Planck-Institute of Biophysics, Frankfurt, and University of Cologne.Google Scholar
  8. 8.
    A substantial part of this study is from the Ph.D. thesis of P. Buet (2000), Max-Planck-Institute of Biophysics, Frankfurt, CNRS Strasbourg, and University of Strasbourg.Google Scholar
  9. 9.
    E. Grell, B. Gersch, P. Buet, G. Quinkert, and J.-M. Lehn, manuscript in preparation.Google Scholar
  10. 10.
    B. Gersch (1998) Ph.D. thesis, Max-Planck-Institute of Biophysics, Frankfurt, and University of Frankfurt.Google Scholar
  11. 11.
    J. R. Lakowicz (1986) Principles of Fluorescence Spectroscopy, Plenum Press, New York, pp. 40-43.Google Scholar
  12. 12.
    R. Arnek, L. G. Sillen, and O. Wahlberg (1968) Ark. Kemi 31, 353-363.Google Scholar
  13. 13.
    P. Brauner, L. G. Sillen, and R. Whiteker (1968) Ark. Kemi 31, 365-376.Google Scholar
  14. 14.
    L. G. Sillen and B. Warnqvist (1968) Ark. Kemi 31, 377-390.Google Scholar
  15. 15.
    A. Schmid, H. Ruf, G. Mager, and E. Stelzer, unpublished program, Max-Planck-Institute of Biophysics, Frankfurt.Google Scholar
  16. 16.
    Microcal Software, Northampton, MA.Google Scholar
  17. 17.
    P. Buet, E. Lewitzki, E. Grell, A.-M. Albrecht-Gary, K. J. Wannovius, F. Maß, H. Elias, A. A. Mundt, and Y. Dupont (2001) Anal. Chem. 73, 857-863.Google Scholar
  18. 18.
    H. Szmacinski and J. R. Lakowicz (1999) Sensors Actuat. B 60, 8-18.Google Scholar
  19. 19.
    R. Haughland (1996) Handbook of Fluorescent Probes and Research Chemicals 6, Molecular Probes, Eugene, OR, pp. 573-575.Google Scholar
  20. 20.
    M. Doludda, F. Kastenholz, E. Lewitzki, and E. Grell (1996) J. Fluoresc. 6, 159-163.Google Scholar
  21. 21.
    J. M. Lehn and J. P. Sauvage (1975) J. Am. Chem. Soc. 12, 6700-6707.Google Scholar
  22. 22.
    G. Anderegg (1975) Helv. Chim. Acta 58, 1218-1225.Google Scholar
  23. 23.
    P. Krumholz (1951) J. Am. Chem. Soc. 73, 3487-3491.Google Scholar

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  1. 1.Max-Planck-Institute of BiophysicsFrankfurtGermany

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