Chromosome Research

, Volume 10, Issue 4, pp 279–286

Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae

  • M. Mandrioli
Article

DOI: 10.1023/A:1016515607278

Cite this article as:
Mandrioli, M. Chromosome Res (2002) 10: 279. doi:10.1023/A:1016515607278

Abstract

Telomeres of the Mamestra brassica holocentric chromosomes were studied by Southern blotting, in-situ hybridization and Bal31 assay evidencing the presence of the telomeric (TTAGG)n repeat. Successively, molecular analysis of telomeres showed that TRAS1 transposable elements were present at the subtelomeric regions of autosomes but not in the NOR-bearing telomeres of the Z and W sex chromosomes. TRAS1 appeared to be transcriptionally active and non-methylated, as evaluated by RT-PCR and digestion with MspI and HpaII. Finally, dot-blotting experiments showed that the 2.8 ± 0.5% of the M. brassicae genome consists of TRAS1.

Bal31 holocentric chromosome Lepidoptera Mamestra brassicae telomere TRAS1 

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M. Mandrioli
    • 1
  1. 1.Dipartimento di Biologia AnimaleUniversità di Modena e Reggio EmiliaModenaItaly

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