, Volume 11, Issue 4, pp 233–241 | Cite as

K-ras oncogene DNA Sequences in Pink Salmon in Streams Impacted by the Exxon Valdez Oil Spill: No Evidence of Oil-induced Heritable Mutations

  • Matthew A. CroninEmail author
  • Jeffrey K. Wickliffe
  • Yelena Dunina
  • Robert J. Baker


It was hypothesized in previous studies that the Exxon Valdez oil spill in Prince William Sound, Alaska, induced heritable mutations and resulted in mortality of pink salmon (Oncorhynchus gorbuscha) embryos. In one of these studies, laboratory exposure of pink salmon embryos to crude oil resulted in apparent mutation-induction in exon 1 and exon 2 of the K-ras oncogene, but no fish from the area impacted by the oil spill were analyzed. We assessed K-ras exon 1 and exon 2 DNA sequences in pink salmon from five streams that were oiled and five streams that were not oiled by the Exxon Valdez oil spill in Prince William Sound, and two streams with natural oil seeps and one stream without seeps on the Alaska Peninsula. Of the 79 fish analyzed for exon 1 and the 89 fish analyzed for exon 2, none had the nucleotide substitutions representing the mutations induced in the laboratory study. Other variable nucleotides occurred in similar proportions in oiled and non-oiled streams and probably represent natural allelic variation. These data do not support the hypothesis that heritable mutations in the K-ras gene were induced by the Exxon Valdez oil spill or oil seeps.

Exxon Valdez oil spill K-ras gene DNA sequences mutations pink salmon 


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  1. Bailey, G.S., Williams, D.E. and Hendricks, J.D. (1996). Fish models for environmental carcinogenesis: The rainbow trout. Environ. Health Perspect. (Suppl. 1), 5–21.Google Scholar
  2. Becker, P.R. and Manen, C.A. (1989). Natural oil seeps in the Alaskan marine environment OCS Study, MMS 89–0065. Final Report. Vol. 62. Outer Continental Shelf Environmental Assessment Program, Minerals Management Service. 126p.Google Scholar
  3. Blasko, D.P. (1976). Occurrences of oil and gas seeps along the Gulf of Alaska. Proceedings of the Eighth Annual Offshore Technology Conference, May 3–6 1976 Vol. 1. Houston, TX, USA.Google Scholar
  4. Brannon, E.L. and Maki, A.W. (1996). The Exxon Valdez oil spill: analysis of impacts on the Prince William Sound pink salmon Rev. Fisheries Sci. 4, 289–337.Google Scholar
  5. Brannon, E.L., Moulton, L.L., Gilbertson, L.G., Maki, A.W. and Skalski, J.R. (1995). An assessment of oil spill effects on pink salmon populations following the Exxon Valdez oil spill Part 1: Early life history. In P.G. Wells, J.N. Butler and J.S. Hughes (eds) Exxon Valdez Oil Spill: Fate and Effects in Alaskan Waters, Philadelphia, PA, USA, ASTM 1219, pp. 548–84.Google Scholar
  6. Bue, B.G., Sharr, S., Moffit, S.D. and Craig, A.K. (1996). Effects of the Exxon Valdez oil spill on pink salmon embryos and preemergent fry. In S.D. Rice, R.B. Spies, D.A. Wolfe, B.A. Wright, Proceedings of the Exxon Valdez Oil Spill. Symposium, Bethesda Maryland, Am. Fish. Soc. Symp. 18, 619–27.Google Scholar
  7. Bue, B.G., Sharr, S. and Seeb, J.E. (1998). Evidence of damage to pink salmon populations inhabiting Prince William Sound, Alaska, two generations after the Exxon Valdez oil spill Trans. Am. Fish. Soc. 127, 35–43.Google Scholar
  8. Cronin, M.A. and Bickham, J.W. (1998). A population genetic analysis of the potential for crude oil spill to induce heritable mutations and impact natural populations Ecotoxicology 7, 259–78.Google Scholar
  9. Fong, A.T., Dashwood, R.H., Cheng, R., Mathews, C., Ford, B., Hendricks, J.D. and Bailey, G.S. (1993). Carinogenicity, metabolism and Ki-ras proto-oncogene activation by 7,12-dimethylbenz[α]anthracene in rainbow trout embryos. Carinogenesis 14, 629–35.Google Scholar
  10. Hendricks, J.D., Cheng, R., Shelton, D.W., Periera, C.B. and Bailey, G.S. (1994). Dose-dependent carcinogenicity and frequent ki-ras proto-oncogene activation by dietary N-nitrosodethyamine in rainbow trout. Fun. Appl. Tox. 23, 53–62.Google Scholar
  11. Jukes, T.H. and Cantor, C.R. (1969). Evolution of protein molecules. In H.N. Munro (ed.) Mammalian Protein Metabolism: New York, NY, USA. Academic Press, pp. 21–132.Google Scholar
  12. Kumar, S., Tamura, K., Jakobsen, I.B. and Nei, M. (2001). MEGA2: Molecular Evolutionary Genetics Analysis software Bioinformatics 17, 1244–1245.Google Scholar
  13. Maki, A.W., Brannon, E.L., Gilbertson, L.G., Moulton, L.L. and Skalski, J.R. (1995). An assessment of oil spill effects on pink salmon populations following the Exxon Valdez oil spill Part 2: adults and escapement. In P.G. Wells, J.N. Butler, and J.S. Hughes (eds) Exxon Valdez Oil Spill: Fate and Effects in Alaskan Water, Philadelphia, PA, USA, ASTM 1219, pp. 585–625.Google Scholar
  14. Miller, G.D., Seeb, J.E. and Bue, B.G. (1994). Saltwater exposure at fertilization induces ploidy alternations, including mosaicism, in salmonids. Can. J. Fish. Aquatic. Sci. 51, 42–49.Google Scholar
  15. Roy, N.K., Stabile, J., Seeb, J.E., Habicht, C. and Wirgin, I. (1999). High Frequency of K-ras mutations in pink salmon embryos experimentally exposed to Exxon Valdez oil. Environ. Toxicol. Chem. 18, 1521–8.Google Scholar
  16. Seeb, J.E., Habicht, C., Greene, B., Kretschmer, E., Olsen, J.B. and Evans, D. (1996). Laboratory examination of oil-related embryo mortalities that persist in pink salmon populations in Prince William Sound. Exxon Valdez Oil Spill Restoration Project Annual Report (Restoration Project 95191A-2). Alaska Department of Fish and Game Anchorage AK.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Matthew A. Cronin
    • 1
    Email author
  • Jeffrey K. Wickliffe
    • 2
  • Yelena Dunina
    • 2
  • Robert J. Baker
    • 2
  1. 1.LGL Alaska Research AssociatesAnchorageUSA;
  2. 2.Department of Biological SciencesTexas Tech UniversityLubbockUSA

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