Russian Journal of Plant Physiology

, Volume 49, Issue 4, pp 541–544 | Cite as

Induction of Phytohemagglutinin Activity by Arachidonic Acid in in vitro Potato Plants

  • N. A. Rozhnova
  • G. A. Gerashenkov
  • A. B. Babosha


Tube-grown potato (Solanum tuberosum L., cv. Nevskii) plants treated with arachidonic acid (AA) were used as a model to study the activity of phytohemagglutinins (PHA) during induction of the plant antiviral defense system. Plant treatment with 10–8 M AA and also their inoculation with potato viruses X, Y, and M resulted in the increased activity of PHAs in potato shoots. The inducer of antiviral resistance behaved as a modulator of the PHA activity providing for its various levels during the development of viral infection. During the development of AA-induced systemic resistance, the level of phytohemagglutinin activity did not essentially depend on the nature of the viral pathogen. We suggested that the mechanism of AA-induced plant antiviral defense was connected with changes in the PHA activity.

Solanum tuberosum arachidonic acid phytohemagglutinins (lectins) phytoviruses antiviral resistance 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Tolstikov, G.A., Miftakhov, M.S., Lazareva, D.N., Pomoinetskii, V.D., and Sidorov, N.N., Prostaglandiny i ikh analogi v reproduktsii zhivotnykh i cheloveka (Prostaglandins and Their Analogs in the Mammal and Human Reproduction), Ufa: Ufimskh. Nauchn. Tsentr, Ural. Otd. Akad. Nauk SSSR, 1989.Google Scholar
  2. 2.
    Kogteva, G.S. and Bezuglov, V.V., Unsaturated Fatty Acids as Endogenous Bioregulators, Biokhimiya, 1998, vol. 63, pp. 6–15.Google Scholar
  3. 3.
    Samuelsson, B., Dahlen, S.E., Lindgren, J., Rouzer, C.A., and Serhan, C.N., Leucotrienes and Lipoxins: Structures, Biosynthesis, and Biological Effects, Science, 1987, vol. 237, pp. 1171–1176.Google Scholar
  4. 4.
    Firsov, D.L., Parnova, R.G., and Natochin, Yu.V., Free Arachidonic Acid as Messenger and Modulator in the Mechanism of Hydroosmotic Effect of Vasopressin, Dokl. Akad. Nauk, 1992, vol. 325, pp. 1252–1254.Google Scholar
  5. 5.
    Trofimets, L.N., Ozeretskovskaya, O.L., Gilyazetdinov, Sh.Y., Balakhontsev, E.N., Yanishevskii, L.V., and Mardanshin, I.S., Inducer of Resistance to Viral Infections in Solonaceae, Inventor's Certificate no. 2072779 (Russia), Byull. Izobret., 1997, no. 4, p. 143.Google Scholar
  6. 6.
    Ladygina, M.E. and Babosha, A.V., Physiological and Biochemical Mechanisms Governing Viral Pathogenesis and Plant Antiviral Activity, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 729–742 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  7. 7.
    Scheggia, C., Prisco, A.E., Dey, P.M., Daleo, G.R., and Lezica, R.P., Alteration of Lectin Pattern in Potato Tuber by Virus, Plant Sci., 1988, vol. 58, pp. 9–14.Google Scholar
  8. 8.
    Zhuk, I.P., Culture of Plant Cells and Tissues in Molecular-Biological and Applied Investigations, S-kh. Biol., 1987, no. 8, pp. 57–62.Google Scholar
  9. 9.
    Griffiths, H.M. and Slack, S.A., Effect of Chemical and Heat Therapy on Virus Concentration in vitro Potato Plantlets, Can. J. Bot., 1990, vol. 68, pp. 1515–1521.Google Scholar
  10. 10.
    Kohm, B.A., Goulden, M.G., Gilbert, J.E., Kavanagh, T.A., and Baulcombe, D.C., A Potato Virus X Resistance Gene Mediates an Induced, Nonspecific Resistance in Protoplasts, Plant Cell, 1993, vol. 5, pp. 913–920.Google Scholar
  11. 11.
    Petrovic, N., Ravnikar, M., and Gogala, N., Interactions between Potato Mosaic Virus and Methyl Jasmonate in Potato, Acta Pharm., 1995, vol. 45, pp. 289–294.Google Scholar
  12. 12.
    Chirkov, S.N., Surgucheva, N.A., and Atabekov, I.G., Stimulation of Cell Protein Synthesis and Inhibition of Viral Infection by Chitosan in Isolated Tobacco Protoplasts, Dokl. Akad. Nauk, 1995, vol. 341, pp. 836–838.Google Scholar
  13. 13.
    Anisimov, B.V., Musin, S.M., Trofimets, L.N., and Aleksashova, M.V., Sorta kartofelya, vozdelyvaemye v Rossiiskoi Federatsii (Katalog) (Potato Cultivars Grown in the Russia, Catalogue), Moscow: Informagrotekh, 1993.Google Scholar
  14. 14.
    Trofimets, L.N., Boiko, V.V., Anisimov, B.V., Knyazeva, V.P., Fenina, N.A., Gurov, V.A., Rusinova, A.Ya., Musin, S.M., Ostapenko, D.P., Reznik, V.S., Puzankov, O.P., Gaiduk, V.N., Ragozina, I.I., and Atabekov, I.G., Bezvirusnoe semenovodstvo kartofelya (rekomendatsii) (The Production of Virus-Free Potato Seed Tubers, Recommendations), Moscow: Agropromizdat, 1990.Google Scholar
  15. 15.
    Rozhnova, N.A., Gerashchenkov, G.A., Yanina, M.M., and Gilyazetdinov, Sh.Y., Emistim Is an Inducer of Resistance to Viral Diseases in Solonaceae, Agrarnaya Rossiya, 1999, no. 1, pp. 35–38.Google Scholar
  16. 16.
    Babosha, A.V. and Ladygina, M.E., Determination of Phytohemagglutinin as Related to Virus Tolerance of Potato Plants, Fiziologo-biokhimicheskie i biofizicheskie metody diagnostiki stepeni ustoichivosti rastenii k patogenam i drugim faktoram (Physiological, Biochemical, and Biophysical Methods for Diagnostics of Plant Resistance to Pathogens and Other Factors), Ladygina, M.E., Ed., Moscow: Moscow Gos. Univ., 1992, pp. 43–52.Google Scholar
  17. 17.
    Rozhnova, N.A., Gerashchenkov, G.A., Odintsova, T.I., Musin, S.M., and Pukhal'skii, V.A., Protective Effect of Arachidonic Acid during Viral Infection: Synthesis of New Proteins by in vitro Potato Plants, Fiziol. Rast. (Moscow), 2001, vol. 48, pp. 897–905 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  18. 18.
    Babosha, A.V., Effect of Human α-Interferon and Virus Infection on the Phytohemagglutinin Activity and Some Other Responses in Tobacco and Potato Leaves, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 891–898 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  19. 19.
    Bostock, R.M., Kuc, J., and Laine, R.A., Eicosapentaenoic and Arachidonic Acids from Phytophthora infestans Elicit Fungitoxic Sesquiterpenes in the Potato, Science, 1981, vol. 212, pp. 67–69.Google Scholar
  20. 20.
    Bostock, R.M., Laine, R.A., and Kuc, J.A., Factors Affecting the Elicitation of Sesquiterpenoid Phytoalexin Accumulation by Eicosapentaenoic and Arachidonic Acids in Potato, Plant Physiol., 1982, vol. 70, pp. 1417–1424.Google Scholar
  21. 21.
    Stermer, B.A. and Bostock, R.M., Rapid Changes in Protein, Synthesis after Application of Arachidonic Acid to Potato Tuber Tissue, Physiol. Mol. Plant Pathol., 1989, vol. 35, pp. 347–356.Google Scholar
  22. 22.
    Ozeretskovskaya, O.L., Chalova, L.I., Avdyushko, S.A., Chalenko, G.I., and Karavaeva, K.A., Eicosanoids as Inducers of Increased Resistance of Potato Plants to Late Blight, Fiziol. Rast. (Moscow), 1988, vol. 35, pp. 175–183 (Sov. Plant Physiol., Engl. Transl.).Google Scholar
  23. 23.
    Ozeretskovskaya, O.L. and Chalova, L.I., Induction of Plant Resistance to Diseases, Molekulyarnye i geneticheskie mekhanizmy vzaimodeistviya mikroorganizmov s rasteniyami (Molecular and Genetical Mechanisms of Interaction between Microorganisms and Plants), Baev, A.A. and Bur'yanov, Ya.I., Eds., Pushchino: Nauchn. Tsentr Biol. Issled. Akad. Nauk SSSR, 1989, pp. 178–184.Google Scholar
  24. 24.
    Molina, A., Gorlach, J., Volrath, S., and Ryals, J., Wheat Genes Encoding Two Types of PR-1 Proteins Are Pathogen Inducible, but Do Not Respond to Activators of Systemic Acquired Resistance, Mol. Plant-Microbe Interact., 1999, vol. 12, pp. 53–58.Google Scholar
  25. 25.
    Penninckx, I.A., Thomma, B.P., Buchala, A., Metraux, J.P., and Broekaert, W.F., Concomitant Activation of Jasmonate and Ethylene Response Pathways Is Required for Induction of a Plant Defensin Gene in Arabidopsis, Plant Cell, 1998, vol. 10, pp. 2103–2113.Google Scholar
  26. 26.
    Pieterse, C.M.J., van Wees, S.C.M., van Pelt, J.A., Knoester, M., Laan, R., Gerrits, H., Weisbeek, P.J., and van Loon, L.C., A Novel Signaling Pathway Controlling Induced Systemic Resistance in Arabidopsis, Plant Cell, 1998, vol. 10, pp. 1571–1580.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2002

Authors and Affiliations

  • N. A. Rozhnova
    • 1
  • G. A. Gerashenkov
    • 1
  • A. B. Babosha
    • 2
  1. 1.Institute of Biochemistry and Genetics, Ufa Research CenterRussian Academy of SciencesUfa, BashkortostanRussia
  2. 2.Main Botanical GardenRussian Academy of SciencesMoscow

Personalised recommendations