Pharmaceutical Research

, Volume 12, Issue 11, pp 1623–1627

Iontophoresis Enhances the Transport of Acyclovir Through Nude Mouse Skin by Electrorepulsion and Electroosmosis

  • Nadia Maria Volpato
  • Patrizia Santi
  • Paolo Colombo
Article

Abstract

Purpose. Iontophoresis was employed for enhancing the transdermal delivery of acyclovir through nude mouse skin in vitro, with the aim of understanding the mechanisms responsible for drug transport, in order to properly set the conditions of therapeutical application.

Methods. Experiments were done in horizontal diffusion cells, using as donor a saturated solution of acyclovir at two different pH values (3.0 and 7.4). Different electrical conditions (current density and polarity) were employed.

Results. At pH 3.0, acyclovir anodal transport was due to electrorepulsion, since acyclovir was 20% in the protonated form. In acyclovir anodal iontophoresis at pH 7.4 the main mechanism involved was electroosmosis, since the drug was substantially unionized and the negative charge of the skin at this pH caused the electroosmotic flow to be from anode to cathode. In the case of cathodal iontophoresis at pH 3.0, acyclovir transport was enhanced approx. seven times, due to the presence of an electroosmotic contribution caused by the reversal of the charge of the skin. At pH 7.4 during cathodal iontophoresis acyclovir transport was not enhanced because the electroosmotic flow was in the opposite direction, compared to drug electric transport, i.e. anode to cathode. The increased skin permeability caused by current application was demonstrated to be less important than electrorepulsion and electroosmosis.

Conclusions. Anodal iontophoresis shows potential applicability for enhancing acyclovir transport to the skin, considering that both electric transport and electroosmosis can be used by appropriately setting the pH of the donor.

iontophoresis acyclovir electroosmosis electrorepulsion transdermal delivery nude mouse skin 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Nadia Maria Volpato
    • 1
  • Patrizia Santi
    • 2
  • Paolo Colombo
    • 2
  1. 1.Departamento de Tecnologia Farmacêutica, Faculdade de FarmáciaUFRJRio de JaneiroBrazil
  2. 2.Pharmaceutical DepartmentUniversity of ParmaParmaItaly

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