Biodiversity & Conservation

, Volume 11, Issue 8, pp 1469–1477 | Cite as

In vitro propagation of Stackhousia tryonii Bailey (Stackhousiaceae): a rare and serpentine-endemic species of central Queensland, Australia

  • Poonam Bhatia
  • Naveen P. Bhatia
  • Nanjappa Ashwath


Stackhousia tryonii Bailey, a rare species whichhyperaccumulates nickel and with a potential to be exploited inphytoremediation/phytomining, is difficult to propagate via seeds. This studyinvestigated the development of a micropropagation protocol for the productionof large stocks of S. tryonii. Disinfested shoot tips andnodal buds were precultured on Gamborg's (B5) basal medium toobtain aseptic shoots for the optimisation of the protocol. 6-Benzyl aminopurine(BAP) at 1.0 mg l−1 produced the highest number ofshoots per explant in B5 medium. Comparison betweenB5 and MS media showed similar responses, but with marked influenceof BAP concentration on shoot numbers. Transfer of shoots from MS(multiplication) medium to MS medium supplemented with indole-3-acetic acid(IAA) and indole-3-butyric acid (IBA), individually or in combination, indicatedthat a combination of IAA and IBA (0.75 mg l−1each) is required to produce roots on young shoots (75%) compared to IBA(15–45%) or IAA (0–10%) alone. This study demonstrated that by usingthis protocol, a high multiplication rate (up to 18 shoots per explant) could be produced within 4 weeks, andthey can be readily hardened (98% survival) in a glasshouse by transplantingthem into a potting mixture of sand and perlite (4:1).

Conservation In vitro culture Micropropagation Nickel hyperaccumulator Phytomining Phytoremediation Rare species Serpentine habitat Stackhousiaceae Stackhousia tryonii Bailey 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Poonam Bhatia
    • 1
  • Naveen P. Bhatia
    • 1
  • Nanjappa Ashwath
    • 1
  1. 1.School of Biological and Environmental SciencesCentral Queensland UniversityRockhamptonAustralia

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