Biologia Plantarum

, Volume 45, Issue 3, pp 347–351 | Cite as

Direct Differentiation of Shoot Buds from Leaf Explants of Cajanus Cajan L.

  • P. Misra


A protocol was developed for direct differentiation of multiple shoot buds from leaf explants of Cajanus cajan. In a modified Murashige and Skoog's medium supplemented with 2.22 µM benzyladenine (BA), 0.57 µM indole-3-acetic acid (IAA) and 41 µM adenine sulphate (AdS), the segments of basal halves of the first two leaves of a young seedling incubated on filter paper bridges in liquid medium took 20 – 25 d to differentiate shoot buds. The explants after transfer to solidified medium, with lower concentration of BA (0.22 μM) resulted in fast growing healthy shoots. The developed shoots (measuring ca. 3 cm) were rooted in a medium supplemented with 1.42 µM IAA. They were subsequently grown in pots with soil with more than 80 % transplantation success.

agar medium liquid medium pigeon pea plant regeneration histology 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • P. Misra
    • 1
  1. 1.Tissue Culture LaboratoryNational Botanical Research InstituteLucknowIndia

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