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Angiogenesis

, Volume 4, Issue 4, pp 269–275 | Cite as

Fibrin fragment E stimulates the proliferation, migration and differentiation of human microvascular endothelial cells in vitro

  • C.A. Bootle-Wilbraham
  • S. Tazzyman
  • W.D. Thompson
  • C.M. Stirk
  • C.E. Lewis
Article

Abstract

Various factors involved in haemostasis also regulate the development of new blood vessels by a process called angiogenesis. Enzymatic cleavage of fibrin yields a variety of fibrin degradation products, particularly in areas of intense angiogenesis such as in healing wounds and active atherosclerotic plaques. One of these, fibrin fragment E (FnE), is a potent angiogenic factor in the chick chorioallantoic membrane assay of angiogenesis. Here, we extend these studies to show that FnE stimulates the proliferation, migration and differentiation of human dermal microvascular endothelial cells (HuDMECs) in vitro, both in the absence and presence of such additional endothelial growth factors as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). We also show that these stimulatory effects occur at concentrations of the protein known to be present in angiogenic tissues in vivo. FnE enhanced the angiogenic effects of VEGF or bFGF, indicating a possible synergy between the signalling pathways used by these three angiogenic factors.

angiogenesis endothelial cell fibrin fibrinolysis fragment E Matrigel 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • C.A. Bootle-Wilbraham
    • 1
  • S. Tazzyman
    • 1
  • W.D. Thompson
    • 2
  • C.M. Stirk
    • 3
  • C.E. Lewis
    • 4
  1. 1.Tumour Targeting Group, Section of Pathology, Division of Genomic MedicineUniversity of Sheffield Medical SchoolSheffieldUK
  2. 2.Department of PathologyUniversity of Aberdeen Medical School, Aberdeen Royal InfirmaryAberdeenUK
  3. 3.Molecular and Cell BiologyUniversity of Aberdeen Medical School, Aberdeen Royal InfirmaryAberdeenUK
  4. 4.Tumour Targeting Group, Section of Pathology, Division of Genomic MedicineUniversity of Sheffield Medical SchoolSheffieldUK. Tel/Fax:

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