Biomedical Microdevices

, Volume 4, Issue 3, pp 213–221 | Cite as

“Smart” Materials for Biosensing Devices: Cell-Mimicking Supramolecular Assemblies and Colorimetric Detection of Pathogenic Agents

  • Jie Song
  • Quan ChengEmail author
  • Shimin Zhu
  • Raymond C. Stevens


Mimicking cell membrane and the biomolecular recognition associated with membranes represents a great technical challenge, yet it has opened doors to innovative diagnostic and therapeutic methods. Our work has focused on design and synthesis of a class of “smart materials” exploiting biological principals for use in biosensors: these materials are functional polymeric assemblies that mimic the cell membrane and conveniently report the presence of pathogens with a color change. Biologically active cell membrane components are incorporated into conjugated polymers with desirable optical properties and the binding of the target molecules onto the material triggers conformational and electronic shifts that are reflected in a chromatic change (a so-called biochromic shift) that is conveniently observed and recorded. Langmuir–Blodgett thin films and vesicle bilayers provide ideal configurations for precise delivery of the biological binding entity to the sensing interface, and for control of molecular orientation for effective biomolecular interaction. Polydiacetylenic membrane-mimicking materials containing cell surface receptor gangliosides and sialic acid residues, respectively were formulated into these architectures and used for colorimetric detection of bacterial toxins and influenza virus. One advantage of these biochromic conjugated polymer (BCP) sensors is that their molecular recognition and signal transduction functionalities are resident in a single functional unit, making them amenable to convenient microfabrication and use.

colorimetric biosensors supramolecular assembly biochromic conjugated polymer molecular recognition cell mimicking pathogen detection 


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  1. D. Charych and J.O. Nagy, CHEMTECH, 26, 24 (1996).Google Scholar
  2. D.H. Charych, J.O., Nagy, W. Spevak, and M.D. Bednarski, Science 261, 585 (1993).Google Scholar
  3. D. Charych, Q. Cheng, A. Reichert, G. Kuziemko, M. Stroh, J.O. Nagy, W. Spevak, and R.C. Stevens, Chem. Biol. 3, 113 (1996).Google Scholar
  4. Q. Cheng and R.C. Stevens, Adv. Mater. 9, 481 (1997).Google Scholar
  5. Q. Cheng and R.C. Stevens, Langmuir 14, 1974 (1998).Google Scholar
  6. Q. Cheng, M. Yamamoto, and R.C. Stevens, Langmuir 16, 5333 (2000).Google Scholar
  7. K. Faid and M. Leclerc, J. Am. Chem. Soc. 120, 5274 (1998).Google Scholar
  8. G.M. Kuziemko, M. Dtroh, and R.C. Stevens, Biochemistry 35, 6375 (1996).Google Scholar
  9. M. Leclerc, Adv. Mater. 11, 1491 (1999).Google Scholar
  10. Z. Ma, J. Li, M. Liu, J. Cao, Z. Zou, J. Tu, and L. Jiang, J. Am. Chem. Soc. 120, 12678 (1998).Google Scholar
  11. J.O. Nagy and M.D. Bednaski, Tet. Lett. 32, 3953 (1991).Google Scholar
  12. R.E. Newnham and A. Amin, CHEMTECH 29, 38 (1999).Google Scholar
  13. S.Y. Okada, R. Jelinek, and D. Charych, Angew. Chem. Int. Ed. Engl. 38, 655 (1999).Google Scholar
  14. S. Okada, S. Peng, W. Spevak, and D. Charych, Acc. Chem. Res. 31, 229 (1998).Google Scholar
  15. B.J. Orchard and S.K. Tripathy, Macromolecules 19, 1844 (1986).Google Scholar
  16. R. Rappuoli and C. Montecucco (eds), Guidebook to Protein Toxins and Their Use in Cell Biology (Oxford University Press, Oxford, 1997).Google Scholar
  17. H. Ringsdorf, B. Schlarb, and J. Venzmer, Angew. Chem. Int. Ed. Engl. 27, 113 (1988).Google Scholar
  18. T.K. Sixma, S.E. Pronk, K.H. Kalk, E.S. Wartna, B.A.M. Vanzanten, B. Witholt, and W.G.J. Hol, Nature 351, 371 (1991).Google Scholar
  19. T. Skotheim, J.R. Reynolds, and R.L. Elsenbaumer (eds), Handbook of Conducting Polymers, 2nd edn (Marcel Dekker, New York, 1997).Google Scholar
  20. J. Song, Q. Cheng, S. Kopta, and R.C. Stevens, J. Am. Chem. Soc. 123, 3205 (2001).Google Scholar
  21. W. Spevak, J.O. Nagy, D.H. Charych, M.E. Schaefer, J.H. Gilbert, and M.D. Bednarski, J. Am. Chem. Soc. 115, 1146 (1993).Google Scholar
  22. L. Svennerholm, Life Sci. 55, 2125 (1994).Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Jie Song
    • 1
  • Quan Cheng
    • 1
    Email author
  • Shimin Zhu
    • 1
  • Raymond C. Stevens
    • 1
    • 2
  1. 1.Materials Sciences Division, Lawrence Berkeley National LaboratoryUniversity of CaliforniaBerkeley
  2. 2.Department of Chemistry and Molecular BiologyThe Scripps Research InstituteLa Jolla

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