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Pharmaceutical Research

, Volume 5, Issue 8, pp 528–532 | Cite as

Formulation of Vaccine Adjuvant Muramyldipeptides (MDP). 2. The Thermal Reactivity and pH of Maximum Stability of MDP Compounds in Aqueous Solution

  • Michael F. Powell
  • Linda C. Foster
  • Allyn R. Becker
  • William Lee
Article

Abstract

The degradation of muramyldipeptides (MDPs) in aqueous solution obeys the rate law kobs = kHH+ + ko + kHO-aHO- and the Arrhenius equation. For example, the rate constants for degradation of N-acetylmuramyl-L-threonyl-D-isoglutamine, 3, at 25°C are kH+ = 2.3 × 10−6M−1 sec−1, ko = 8.2× 10−10 sec−1, and kHO− = 0.19 M−l sec−1. The degradation rates are dependent on the side-chain substituents; it is predicted that sterically hindered MDP compounds will show an extended shelf life in aqueous solution. Product studies in the weakly acid pH region (where the pH of maximum stability occurs) show that MDP compounds degrade largely by hydrolysis of the dipeptide side chain. These data show that MDP 3 exhibits a shelf life (t90) of greater than 2 years in aqueous solutions of pH 4–4.5, the pH of maximum stability.

muramyldipeptide hydrolysis kinetics parenteral formulation high-pressure liquid chromatography (HPLC) 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Michael F. Powell
    • 1
    • 2
  • Linda C. Foster
    • 1
    • 3
  • Allyn R. Becker
    • 1
  • William Lee
    • 1
  1. 1.Institute of Pharmaceutical SciencesSyntex ResearchPalo Alto
  2. 2.Cytel CorporationSan Diego
  3. 3.California BiotechnologyMountain View

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