Pharmaceutical Research

, Volume 7, Issue 11, pp 1141–1146

Electrical Analysis of Fresh, Excised Human Skin: A Comparison with Frozen Skin

  • Gerald B. Kasting
  • Lisa A. Bowman
Article

Abstract

Samples of human allograft skin prepared without freezing ("fresh skin”) were found to have electrical and sodium ion transport properties which differed only slightly from those of skin which had been similarly treated but stored frozen (“frozen skin”). The fresh skin samples were less permeable to sodium ions during passive diffusion and less conductive than frozen skin at low current levels. They were more permselective for sodium versus chloride during constant-current iontophoresis and showed slightly more asymmetry in their current–voltage properties. Overall, the electrical behavior of the two tissues was similar enough to support the use of frozen tissue in iontophoresis studies. However, caution should be exercised when considering the use of frozen skin for applications, such as those based on electroosmosis, where the observed differences could have a major impact on the results.

iontophoresis human skin current–voltage characteristic sodium ion transport fresh skin frozen skin 

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REFERENCES

  1. 1.
    G. B. Kasting and L. A. Bowman. Pharm. Res. 7:134–143 (1990).Google Scholar
  2. 2.
    G. B. Kasting. E. W. Merritt, and J. C. Keister. J. Membrane Sci. 35:137–159 (1988).Google Scholar
  3. 3.
    R. R. Burnette and B. Ongpipattanakul. J. Pharm. Sci. 76:765–773 (1987).Google Scholar
  4. 4.
    R. T. Tregear. Nature 205:600–601 (1965).Google Scholar
  5. 5.
    R. T. Tregear. J. Invest. Dermatol. 46:16–23 (1966).Google Scholar
  6. 6.
    J. C. Keister and G. B. Kasting. J. Control. Release 4:111–117 (1986).Google Scholar
  7. 7.
    J. C. Keister and G. B. Kasting. The mechanism of iontophoresis, Proceedings of NIH Workshop on Transdermal Delivery of Drugs, Bethesda, MD, May 23–24, 1988.Google Scholar
  8. 8.
    R. R. Burnette and B. Ongpipattanakul. J. Pharm. Sci. 77:132–137 (1988).Google Scholar
  9. 9.
    H. A. Abramson and M. H. Gorin. J. Phys. Chem. 44:1094–1102 (1940).Google Scholar
  10. 10.
    R. J. Scheuplein. In A. Jarrett (ed.), The Physiology and Pathophysiology of the Skin, Vol. 5, Academic Press, London, 1978, p. 1734.Google Scholar
  11. 11.
    S. Grimnes. Acta Derm. Venereol. (Stockh.) 64:93–98 (1984).Google Scholar
  12. 12.
    K. Hashimoto. In A. Jarrett (ed.), The Physiology and Pathophysiology of the Skin, Vol. 5, Academic Press, London, 1978, pp. 1543–1573.Google Scholar
  13. 13.
    J. O'M. Bockris and A. K. N. Reddy. Modern Electrochemistry, Vol. 2, Plenum Press, New York, 1970, pp. 935–937.Google Scholar
  14. 14.
    J. O'M. Bockris and A. K. N. Reddy. Modern Electrochemistry, Vol. 2, Plenum Press, New York, 1970, Chap. 8, pp. 845–941.Google Scholar
  15. 15.
    A. J. Bard and L. R. Faulkner. Electrochemical Methods: Fundamentals and Applications, Wiley, New York, 1980, Chap. 3, pp. 87–118.Google Scholar
  16. 16.
    L. V. Chernomordik, S. I. Sukharev, I. G. Abidor, and Yu. A. Chizmadzhev. Biochim. Biophys. Acta 736:203–213 (1983).Google Scholar
  17. 17.
    C. J. Bender. Chem. Soc. Rev. 17:317–346 (1988).Google Scholar
  18. 18.
    V. Marecek, Z. Samec, and J. Koryta. Adv. Colloid Interface Sci. 29:1–78 (1988).Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Gerald B. Kasting
    • 1
  • Lisa A. Bowman
    • 1
  1. 1.The Procter & Gamble Company, Miami Valley LaboratoriesCincinnati

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