Liquid Chromatographic Assay and Pharmacokinetics of Quazepam and Its Metabolites Following Sublingual Administration of Quazepam
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A reverse-phase liquid chromatographic method is described for simultaneous quantification of quazepam, and two of its metabolites, 2-oxoquazepam and N-desalkyl-2-oxoquazepam. The method uses a solid-phase extraction procedure to prepare plasma samples. After extraction, the methanolic extract is evaporated; the residue is then reconstituted in a small volume of mobile phase and chromatographed. The total chromatography time for a single sample is about 20 min. A sensitivity of 1 ng/ml for quazepam and its metabolites is attained when 1 ml of plasma is extracted. Analytical recovery of quazepam and its metabolites added to plasma ranged from 87 to 96%. The maximum within-day and day-to-day coefficients of variation for each compound at concentrations of 20 and 60 ng/ml were 7.6 and 11.2%, respectively. The method was applied to sublingual pharmacokinetic studies of quazepam in healthy volunteers.
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- 1.C. P. Robinson. Drugs Today 22:149–157 (1986).Google Scholar
- 2.J. M. Hilbert, M. Chung, G. Maier, R. Gural, S. Symchowicz, and N. Zampaglione. Clin. Pharmacol. Ther. 36:99–104 (1984).Google Scholar
- 3.N. Zampaglione, J. M. Hilbert, J. Ning. M. Chung, R. Gural, and S. Symchowicz. Drug Metab. Dispos. 13:25–29 (1985).Google Scholar
- 4.J. M. Hilbert, J. M. Ning, G. Murphy, A. Jimenez, and N. Zampaglione. J. Pharm. Sci. 73:516–519 (1984).Google Scholar
- 5.H. Bun, P. Coassolo, B. Ba, C. Aubert, and J. Cano. J. Chromatogr. 378:137–145 (1986).Google Scholar
- 6.R. D. Brown and J. E. Manno. J. Pharm. Sci. 67:1687–1691 (1978).Google Scholar
- 7.SAS Institute. SAS User's Guide: Statistics, SAS Institute, Cary, N.C., 1982.Google Scholar