Pharmaceutical Research

, Volume 5, Issue 6, pp 365–368

Liquid Chromatographic Assay and Pharmacokinetics of Quazepam and Its Metabolites Following Sublingual Administration of Quazepam

  • Samir K. Gupta
  • Everett H. EllinwoodJr.
Article

Abstract

A reverse-phase liquid chromatographic method is described for simultaneous quantification of quazepam, and two of its metabolites, 2-oxoquazepam and N-desalkyl-2-oxoquazepam. The method uses a solid-phase extraction procedure to prepare plasma samples. After extraction, the methanolic extract is evaporated; the residue is then reconstituted in a small volume of mobile phase and chromatographed. The total chromatography time for a single sample is about 20 min. A sensitivity of 1 ng/ml for quazepam and its metabolites is attained when 1 ml of plasma is extracted. Analytical recovery of quazepam and its metabolites added to plasma ranged from 87 to 96%. The maximum within-day and day-to-day coefficients of variation for each compound at concentrations of 20 and 60 ng/ml were 7.6 and 11.2%, respectively. The method was applied to sublingual pharmacokinetic studies of quazepam in healthy volunteers.

quazepam metabolites reversed phase high-performance liquid chromatography solid-phase extraction plasma sublingual pharmacokinetics 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Samir K. Gupta
    • 1
  • Everett H. EllinwoodJr.
    • 1
  1. 1.Department of PsychiatryDuke University Medical CenterDurham

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